验证数据展示
经过测试的应用
| Positive WB detected in | MMS treated PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-81740 targets Phospho-CHEK2 (Thr68) in WB applications and shows reactivity with human samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | CHK2 checkpoint homolog (S. pombe) |
| 别名 | |
| 计算分子量 | 61 kDa |
| 观测分子量 | 65 kDa |
| GenBank蛋白编号 | BC004207 |
| 基因名称 | CHEK2 |
| Gene ID (NCBI) | 11200 |
| RRID | AB_3673733 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O96017 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Serine/threonine-protein kinase Chk2 (CHEK2) is a serine/threonine kinase which is activated upon DNA damage and is implicated in pathways that govern DNA repair, cell cycle arrest or apoptosis in response to the initial damage. ATM phosphorylates CHEK2 on T68. Phosphorylation on T68 and subsequent full activation of CHEK2 was shown to require priming phosphorylation on adjacent residues by Polo-like kinase 3 (PLK3) and the dualspecificity tyrosine and serine/threoninekinase TTK/hMPS1. Additionally TTK appears to phosphorylate T68. Phosphorylation of T68 promotes the binding of the N-terminal SQ/TQ-rich cluster of one CHEK2 molecule with the FHA domain of another CHEK2 molecule. (PMID: 28553140, PMID: 18004398, PMID: 33322746)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 Phospho-CHEK2 (Thr68) antibody CL750-81740 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |