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验证数据展示

  • WB analysis of PC-3 using 81740-1-RR (same clone as 81740-1-PBS)

    WB analysis of PC-3 using 81740-1-RR (same clone as 81740-1-PBS)

    Non-treated PC-3 and MMS treated PC-3 cells were subjected to SDS PAGE followed by western blot with 81740-1-RR (Phospho-CHEK2 (Thr68) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control. This data was developed using the same antibody clone with 81740-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 81740-1-RR (same clone as 81740-1-PBS)

    WB analysis of HeLa using 81740-1-RR (same clone as 81740-1-PBS)

    Non-treated HeLa and UV treated HeLa cells were subjected to SDS PAGE followed by western blot with 81740-1-RR (Phospho-CHEK2 (Thr68) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control. This data was developed using the same antibody clone with 81740-1-PBS in a different storage buffer formulation.

  • FC experiment of PC-3 using 81740-1-RR (same clone as 81740-1-PBS)

    FC experiment of PC-3 using 81740-1-RR (same clone as 81740-1-PBS)

    1X10^6 PC-3 cells untreated (dashed lines) or treated with MMS which intracellularly stained with 0.06 ug Phospho-CHEK2 (Thr68) Recombinant antibody (81740-1-RR, Clone:1L2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 81740-1-PBS in a different storage buffer formulation.

Phospho-CHEK2 (Thr68) Recombinant antibody, PBS Only

Phospho-CHEK2 (Thr68) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 81740-1-PBS

产品说明书

CloneNo. 1L2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

CHK2, CHEK2, EC:2.7.11.1, Cds1 homolog, CDS1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CHEK2 Ready-To-Use IHC Kit. CHEK2 primary antibody included.

产品信息

81740-1-PBS targets Phospho-CHEK2 (Thr68) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CHK2 checkpoint homolog (S. pombe)
别名 CHK2, CHEK2, EC:2.7.11.1, Cds1 homolog, CDS1
计算分子量 61 kDa
观测分子量65 kDa
GenBank蛋白编号BC004207
基因名称 CHEK2
Gene ID (NCBI) 11200
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO96017
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Serine/threonine-protein kinase Chk2 (CHEK2) is a serine/threonine kinase which is activated upon DNA damage and is implicated in pathways that govern DNA repair, cell cycle arrest or apoptosis in response to the initial damage. ATM phosphorylates CHEK2 on T68. Phosphorylation on T68 and subsequent full activation of CHEK2 was shown to require priming phosphorylation on adjacent residues by Polo-like kinase 3 (PLK3) and the dualspecificity tyrosine and serine/threoninekinase TTK/hMPS1. Additionally TTK appears to phosphorylate T68. Phosphorylation of T68 promotes the binding of the N-terminal SQ/TQ-rich cluster of one CHEK2 molecule with the FHA domain of another CHEK2 molecule. (PMID: 28553140, PMID: 18004398, PMID: 33322746)