验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, paclitaxel treated Jurkat cells, Calyculin A treated Jurkat cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | Staurosporine treated Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
80771-2-RR targets Phospho-BCL2 (Ser70) in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | B-cell CLL/lymphoma 2 |
| 别名 | BCL-2, BCL2, 230061A8 |
| 计算分子量 | 26 kDa |
| 观测分子量 | 26 kDa |
| GenBank蛋白编号 | BC027258 |
| 基因名称 | BCL2 |
| Gene ID (NCBI) | 596 |
| RRID | AB_3670487 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P10415 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BCL2 belongs to the Bcl-2 family. The anti-apoptotic protein BCL2 can bind to the pro-apoptotic protein BAX or BAK1 to form heterodimers that modulate apoptosis. BCL2 phosphorylation at Ser70 enhances binding to BAX and BAD and is essential for its anti-apoptotic activity. Conversely, BCL2 also regulates autophagy via interaction with BECN1/Beclin 1; moreover, BCL2 phosphorylation including at Ser70 leads to its dissociation from BECN1 and induction of autophagy. These findings suggest that BCL2 regulates the balance between apoptosis and autophagy. (PMID: 29433359)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-BCL2 (Ser70) antibody 80771-2-RR | Download protocol |
| IHC protocol for Phospho-BCL2 (Ser70) antibody 80771-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |