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验证数据展示

  • WB analysis using 80771-2-RR (same clone as 80771-2-PBS)

    WB analysis using 80771-2-RR (same clone as 80771-2-PBS)

    Non-treated Jurkat cells, paclitaxel treated and Calyculin A treated Jurkat cells were subjected to SDS PAGE followed by western blot with 80771-2-RR (Phospho-BCL2 (Ser70) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control. This data was developed using the same antibody clone with 80771-2-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 80771-2-RR (same clone as 80771-2-PBS)

    IHC staining of human tonsillitis using 80771-2-RR (same clone as 80771-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80771-2-RR (Phospho-BCL2 (Ser70) antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80771-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BCL2 antibody included
  • FC experiment of Jurkat using 80771-2-RR (same clone as 80771-2-PBS)

    FC experiment of Jurkat using 80771-2-RR (same clone as 80771-2-PBS)

    1x10^6 Jurkat cells untreated (dashed lines) or treated with Staurosporine which intracellularly stained with 0.06 ug Phospho-BCL2 (Ser70) Recombinant antibody (80771-2-RR, Clone:230061A8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80771-2-PBS in a different storage buffer formulation.

Phospho-BCL2 (Ser70) Recombinant antibody, PBS Only

Phospho-BCL2 (Ser70) Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 80771-2-PBS

产品说明书

CloneNo. 230061A8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

BCL-2, BCL2, 230061A8

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy BCL2 Ready-To-Use IHC Kit. BCL2 primary antibody included.

产品信息

80771-2-PBS targets Phospho-BCL2 (Ser70) in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 B-cell CLL/lymphoma 2
别名 BCL-2, BCL2, 230061A8
计算分子量 26 kDa
观测分子量26 kDa
GenBank蛋白编号BC027258
基因名称 BCL2
Gene ID (NCBI) 596
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP10415
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BCL2 belongs to the Bcl-2 family. The anti-apoptotic protein BCL2 can bind to the pro-apoptotic protein BAX or BAK1 to form heterodimers that modulate apoptosis. BCL2 phosphorylation at Ser70 enhances binding to BAX and BAD and is essential for its anti-apoptotic activity. Conversely, BCL2 also regulates autophagy via interaction with BECN1/Beclin 1; moreover, BCL2 phosphorylation including at Ser70 leads to its dissociation from BECN1 and induction of autophagy. These findings suggest that BCL2 regulates the balance between apoptosis and autophagy. (PMID: 29433359)