验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | λ phosphatase treated HEK-293T cells |
For other applications, we recommend the unconjugated version of this antibody, 80431-2-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-80431-2 targets Phospho-BAD (Ser112) in FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | BCL2-associated agonist of cell death |
| 别名 | BAD (Ser112), p BAD (Ser112), p-BAD, p-BAD (Ser112), p-BAD(Ser112) |
| 计算分子量 | 18 kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | BC001901 |
| 基因名称 | BAD |
| Gene ID (NCBI) | 572 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 激发激光 | Blue laser (488 nm) |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q92934 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BAD, a distant member of the BCL-2 family, exerts its death promoting e€ect by hetero-dimerizing with BCL-XL and BCL-2 death antagonists in the mitochondria. The function of the pro-apoptotic molecule BAD is regulated by phosphorylation of two sites, serine-112 (Ser-112) and serine-136 (Ser-136). Phosphorylation at either site results in loss of the ability of BAD to heterodimerize with the survival proteins BCL-XL or BCL-2. Phosphorylated BAD binds to 14-3-3 and is sequestered in the cytoplasm. (PMID: 10597268)
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 Phospho-BAD (Ser112) antibody CL488-80431-2 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
