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验证数据展示

  • WB analysis of HEK-293 using 80431-2-RR (same clone as 80431-2-PBS)

    WB analysis of HEK-293 using 80431-2-RR (same clone as 80431-2-PBS)

    Non-treated HEK-293 cells, phosphatase inhibitor treated HEK-293 cells and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80431-2-RR (Phospho-BAD (Ser112) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80431-2-PBS in a different storage buffer formulation.

Phospho-BAD (Ser112) Recombinant monoclonal antibody, PBS Only

Phospho-BAD (Ser112) Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 80431-2-PBS

产品说明书

CloneNo. 251074F11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

BAD (Ser112), p BAD (Ser112), p-BAD, p-BAD (Ser112), p-BAD(Ser112)

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

80431-2-PBS targets Phospho-BAD (Ser112) in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 BCL2-associated agonist of cell death
别名 BAD (Ser112), p BAD (Ser112), p-BAD, p-BAD (Ser112), p-BAD(Ser112)
计算分子量 18 kDa
观测分子量25 kDa
GenBank蛋白编号BC001901
基因名称 BAD
Gene ID (NCBI) 572
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ92934
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BAD, a distant member of the BCL-2 family, exerts its death promoting e€ect by hetero-dimerizing with BCL-XL and BCL-2 death antagonists in the mitochondria. The function of the pro-apoptotic molecule BAD is regulated by phosphorylation of two sites, serine-112 (Ser-112) and serine-136 (Ser-136). Phosphorylation at either site results in loss of the ability of BAD to heterodimerize with the survival proteins BCL-XL or BCL-2. Phosphorylated BAD binds to 14-3-3 and is sequestered in the cytoplasm. (PMID: 10597268)

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