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CoraLite® Plus 488-conjugated PYCR1 Polyclonal antibody
PYCR1 Polyclonal Antibody for FC (Intra), IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
FC (Intra), IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy PYCR1 Ready-To-Use IHC Kit. PYCR1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13108-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-13108 targets PYCR1 in IF, FC (Intra) applications and shows reactivity with human samples.
| Tested Applications | FC (Intra), IF/ICC |
| Tested Reactivity | human |
| Immunogen | PYCR1 fusion protein Ag3764 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | pyrroline-5-carboxylate reductase 1 |
| Synonyms | P5C, P5C reductase 1, P5CR, P5CR 1, PIG45, PP222, PYCR, PYCR1 |
| Calculated Molecular Weight | 319 aa, 33.8 kDa |
| Observed Molecular Weight | 33 kDa, 35 kDa |
| GenBank Accession Number | BC022244 |
| Gene Symbol | PYCR1 |
| Gene ID (NCBI) | 5831 |
| RRID | AB_2919068 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P32322 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa. The MW of PYCR1 is about 33-35 kDa. PYCR1 has 3 isoforms produced by alternative splicing. This antibody may have cross reaction to PYCR2 due to the high homology.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PYCR1 antibody CL488-13108 | Download protocol |
| FC protocol for CL Plus 488 PYCR1 antibody CL488-13108 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
