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验证数据展示

  • WB analysis using 66510-1-Ig (same clone as 66510-1-PBS)

    WB analysis using 66510-1-Ig (same clone as 66510-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66510-1-Ig (PYCR1 antibody) at dilution of 1:3000 incubated at room temperature for 1 hour. This data was developed using the same antibody clone with 66510-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66510-1-Ig (same clone as 66510-1-PBS)

    IHC staining of human breast cancer using 66510-1-Ig (same clone as 66510-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66510-1-Ig (PYCR1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66510-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PYCR1 antibody included
  • IF Staining of HeLa using 66510-1-Ig (same clone as 66510-1-PBS)

    IF Staining of HeLa using 66510-1-Ig (same clone as 66510-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PYCR1 antibody (66510-1-Ig, Clone: 1H2G1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66510-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50786-1

    Cytometric bead array standard curve of MP50786-1

    Cytometric bead array standard curve of MP50786-1, PYCR1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66510-2-PBS. Detection antibody: 66510-1-PBS. Standard:Ag17919. Range: 0.098-100 ng/mL.

PYCR1 Monoclonal antibody, PBS Only (Detector)

PYCR1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66510-1-PBS

产品说明书

CloneNo. 1H2G1

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

P5CR 1, P5CR, P5C reductase 1, P5C, EC:1.5.1.2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PYCR1 Ready-To-Use IHC Kit. PYCR1 primary antibody included.

产品信息

66510-1-PBS targets PYCR1 as part of a matched antibody pair:

MP50786-1: 66510-2-PBS capture and 66510-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PYCR1 fusion protein Ag17919 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 pyrroline-5-carboxylate reductase 1
别名 P5CR 1, P5CR, P5C reductase 1, P5C, EC:1.5.1.2
计算分子量 319 aa, 33.8 kDa
观测分子量 33 kDa, 35 kDa
GenBank蛋白编号BC022244
基因名称 PYCR1
Gene ID (NCBI) 5831
RRIDAB_2881873
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP32322
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa.