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PYCR1 Monoclonal antibody

PYCR1 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat and More (1)

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H2G1

Cat No : 66510-1-Ig

Print datasheet

Synonyms

P5C, P5C reductase 1, P5CR, P5CR 1, PIG45, PP222, PYCR, PYCR1

验证数据展示

  • WB analysis using 66510-1-Ig

    WB analysis using 66510-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66510-1-Ig (PYCR1 antibody) at dilution of 1:3000 incubated at room temperature for 1 hour.

  • IHC staining of human breast cancer using 66510-1-Ig

    IHC staining of human breast cancer using 66510-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66510-1-Ig (PYCR1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PYCR1 antibody included
  • IF Staining of HeLa using 66510-1-Ig

    IF Staining of HeLa using 66510-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PYCR1 antibody (66510-1-Ig, Clone: 1H2G1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, COLO 320 cells, MCF-7 cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PYCR1 Ready-To-Use IHC Kit. PYCR1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66510-1-Ig targets PYCR1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman
Immunogen PYCR1 fusion protein Ag17919 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name pyrroline-5-carboxylate reductase 1
Synonyms P5C, P5C reductase 1, P5CR, P5CR 1, PIG45, PP222, PYCR, PYCR1
Calculated Molecular Weight 319 aa, 33.8 kDa
Observed Molecular Weight 33 kDa, 35 kDa
GenBank Accession NumberBC022244
Gene Symbol PYCR1
Gene ID (NCBI) 5831
RRIDAB_2881873
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP32322
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa.

实验方案

Product Specific Protocols
WB protocol for PYCR1 antibody 66510-1-IgDownload protocol
IHC protocol for PYCR1 antibody 66510-1-IgDownload protocol
IF protocol for PYCR1 antibody 66510-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Med

Metabolism-related lncRNAs signature to predict the prognosis of colon adenocarcinoma

Authors - Yimin Sun
View Article