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验证数据展示

  • FC experiment of human peripheral blood lymphocytes using 98715-1-RR

    FC experiment of human peripheral blood lymphocytes using 98715-1-RR

    1x10^6 human peripheral blood lymphocytes were surface stained with 0.25 ug Anti-Human PTPRJ/CD148 Rabbit RecAb (98715-1-RR, Clone: 252382G6) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human peripheral blood lymphocytes using 98715-1-RR

    FC experiment of human peripheral blood lymphocytes using 98715-1-RR

    1x10^6 human peripheral blood lymphocytes were surface stained with 0.25 ug Anti-Human PTPRJ/CD148 Rabbit RecAb (98715-1-RR, Clone: 252382G6) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • IHC staining of human tonsillitis using 98715-1-RR

    IHC staining of human tonsillitis using 98715-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98715-1-RR (DEP-1/CD148 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity of 98715-1-RR

    Affinity of 98715-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98715-1-RR against Human PTPRJ/CD148 were performed. The affinity constant is 0.649 nM.

Anti-Human PTPRJ/CD148 Rabbit Recombinant Antibody

PTPRJ/CD148 Uni-rAb® Recombinant Antibody for IHC, FC
Cat No. 98715-1-RR

产品说明书

CloneNo. 252382G6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, FC

别名

PTPRJ, CD148, Density enhanced phosphatase 1, Density-enhanced phosphatase 1, DEP 1

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inhuman peripheral blood lymphocytes

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98715-1-RR targets PTPRJ/CD148 in IHC, FC applications and shows reactivity with human samples.

经测试应用 IHC, FC Application Description
经测试反应性 human
免疫原

CatNo: Eg3492

Product name: recombinant human DEP-1/CD148 protein

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 36-975 aa of NM_002843.4

Sequence: AGGTPSPIPDPSVATVATGENGITQISSTAESFHKQNGTGTPQVETNTSEDGESSGANDSLRTPEQGSNGTDGASQKTPSSTGPSPVFDIKAVSISPTNVILTWKSNDTAASEYKYVVKHKMENEKTITVVHQPWCNITGLRPATSYVFSITPGIGNETWGDPRVIKVITEPIPVSDLRVALTGVRKAALSWSNGNGTASCRVLLESIGSHEELTQDSRLQVNISGLKPGVQYNINPYLLQSNKTKGDPLGTEGGLDASNTERSRAGSPTAPVHDESLVGPVDPSSGQQSRDTEVLLVGLEPGTRYNATVYSQAANGTEGQPQAIEFRTNAIQVFDVTAVNISATSLTLIWKVSDNESSSNYTYKIHVAGETDSSNLNVSEPRAVIPGLRSSTFYNITVCPVLGDIEGTPGFLQVHTPPVPVSDFRVTVVSTTEIGLAWSSHDAESFQMHITQEGAGNSRVEITTNQSIIIGGLFPGTKYCFEIVPKGPNGTEGASRTVCNRTVPSAVFDIHVVYVTTTEMWLDWKSPDGASEYVYHLVIESKHGSNHTSTYDKAITLQGLIPGTLYNITISPEVDHVWGDPNSTAQYTRPSNVSNIDVSTNTTAATLSWQNFDDASPTYSYCLLIEKAGNSSNATQVVTDIGITDATVTELIPGSSYTVEIFAQVGDGIKSLEPGRKSFCTDPASMASFDCEVVPKEPALVLKWTCPPGANAGFELEVSSGAWNNATHLESCSSENGTEYRTEVTYLNFSTSYNISITTVSCGKMAAPTRNTCTTGITDPPPPDGSPNITSVSHNSVKVKFSGFEASHGPIKAYAVILTTGEAGHPSADVLKYTYEDFKKGASDTYVTYLIRTEEKGRSQSLSEVLKYEIDVGNESTTLGYYNGKLEPLGSYRACVAGFTNITFHPQNKGLIDGAESYVSFSRYSDAVSLPQDPGVICG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, J
别名 PTPRJ, CD148, Density enhanced phosphatase 1, Density-enhanced phosphatase 1, DEP 1
计算分子量146 kDa
GenBank蛋白编号NM_002843.4
基因名称 PTPRJ
Gene ID (NCBI) 5795
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ12913-1
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

PTPRJ, also named as CD148 and DEP1, belongs to the protein-tyrosine phosphatase family and Receptor class 3 subfamily. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPRJ may contribute to the mechanism of contact inhibition of cell growth. PTPRJ is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 (PLCG1) and Linker for Activation of T Cells (LAT). It was also found to dephosphorylate PDGF beta receptor, and may be involved in UV-induced signal transduction.

实验方案

Product Specific Protocols
FC protocol for PTPRJ/CD148 antibody 98715-1-RRDownload protocol
IHC protocol for PTPRJ/CD148 antibody 98715-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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