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验证数据展示

  • IF Staining of mouse brain using CL594-66921

    IF Staining of mouse brain using CL594-66921

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 PTGER4 antibody (CL594-66921, Clone: 4A2A12 ) at dilution of 1:200.

  • IF Staining of mouse brain using CL594-66921

    IF Staining of mouse brain using CL594-66921

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 PTGER4 antibody (CL594-66921, Clone: 4A2A12 ) at dilution of 1:200.

  • IF Staining of mouse skin using CL594-66921

    IF Staining of mouse skin using CL594-66921

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using CoraLite®594 PTGER4 antibody (CL594-66921, Clone: 4A2A12 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CoraLite®594-conjugated PTGER4 Monoclonal antibody

PTGER4 Monoclonal Antibody for IF-P
Cat No. CL594-66921

产品说明书

CloneNo. 4A2A12

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig

应用

IF-P

别名

4A2A12, EP4, EP4R

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF-P detected inmouse brain tissue, mouse skin tissue
For other applications, we recommend the unconjugated version of this antibody, 66921-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66921 targets PTGER4 in IF-P applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag19262

Product name: Recombinant human PTGER4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 333-488 aa of BC101534

Sequence: RKTVLSKAIEKIKCLFCRIGGSRRERSGQHCSDSQRTSSAMSGHSRSFISRELKEISSTSQTLLPDLSLPDLSENGLGGRNLLPGVPGMGLAQEDTTSLRTLRISETSDSSQGQDSESVLLVDEAGGSGRAGPAPKGSSLQVTFPSETLNLSEKCI

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 prostaglandin E receptor 4 (subtype EP4)
别名 4A2A12, EP4, EP4R
计算分子量 488 aa, 53 kDa
观测分子量 53 kDa
GenBank蛋白编号BC101534
基因名称 PTGER4
Gene ID (NCBI) 5734
RRIDAB_2920056
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP35408
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL594 PTGER4 antibody CL594-66921Download protocol
Standard Protocols
Click here to view our Standard Protocols
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