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验证数据展示

  • FC experiment of HepG2 using CL488-66921

    FC experiment of HepG2 using CL488-66921

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human PTGER4 (CL488-66921, Clone:4A2A12) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2b Isotype Control (MPC-11) (CL488-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated PTGER4 Monoclonal antibody

PTGER4 Monoclonal Antibody for FC (Intra)
Cat No. CL488-66921

产品说明书

CloneNo. 4A2A12

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse, Rat, Pig

应用

FC (Intra)

别名

PTGER4, 4A2A12, EP4, EP4R, PTGER2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66921-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66921 targets PTGER4 in FC (Intra) applications and shows reactivity with Human, Mouse, Rat, Pig samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, Mouse, Rat, Pig
免疫原

CatNo: Ag19262

Product name: Recombinant human PTGER4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 333-488 aa of BC101534

Sequence: RKTVLSKAIEKIKCLFCRIGGSRRERSGQHCSDSQRTSSAMSGHSRSFISRELKEISSTSQTLLPDLSLPDLSENGLGGRNLLPGVPGMGLAQEDTTSLRTLRISETSDSSQGQDSESVLLVDEAGGSGRAGPAPKGSSLQVTFPSETLNLSEKCI

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 prostaglandin E receptor 4 (subtype EP4)
别名 PTGER4, 4A2A12, EP4, EP4R, PTGER2
计算分子量 488 aa, 53 kDa
观测分子量 53 kDa
GenBank蛋白编号BC101534
基因名称 PTGER4
Gene ID (NCBI) 5734
RRIDAB_3084288
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP35408
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 PTGER4 antibody CL488-66921Download protocol
Standard Protocols
Click here to view our Standard Protocols