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验证数据展示

  • WB analysis using 67462-1-Ig (same clone as 67462-1-PBS)

    WB analysis using 67462-1-Ig (same clone as 67462-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67462-1-Ig (PTBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67462-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67462-1-Ig (same clone as 67462-1-PBS)

    IHC staining of human colon cancer using 67462-1-Ig (same clone as 67462-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67462-1-Ig (PTBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67462-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PTBP1 antibody included
  • IF Staining of A375 using 67462-1-Ig (same clone as 67462-1-PBS)

    IF Staining of A375 using 67462-1-Ig (same clone as 67462-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using 67462-1-Ig (PTBP1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67462-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67462-1-Ig (same clone as 67462-1-PBS)

    WB analysis of HeLa using 67462-1-Ig (same clone as 67462-1-PBS)

    WB result of PTBP1 antibody (67462-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTBP1 transfected HeLa cells. This data was developed using the same antibody clone with 67462-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PTBP1 Monoclonal antibody, PBS Only

PTBP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67462-1-PBS

产品说明书

CloneNo. 3D6D8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

3D6D8, HNRNP I, HNRNPI, HNRPI, pPTB

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PTBP1 Ready-To-Use IHC Kit. PTBP1 primary antibody included.

产品信息

67462-1-PBS targets PTBP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag28404

Product name: Recombinant human PTBP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-370 aa of BC013694

Sequence: MDGIVPDIAVGTKRGSDELFSTCVTNGPFIMSSNSASAANGNDSKKFKGDSRSAGVPSRVIHIRKLPIDVTEGEVISLGLPFGKVTNLLMLKGKNQAFIEMNTEEAANTMVNYYTSVTPVLRGQPIYIQFSNHKELKTDSSPNQARAQAALQAVNSVQSGNLALAASAAAVDAGMAMAGQSPVLRIIVENLFYPVTLDVLHQIFSKFGTVLKIITFTKNNQFQALLQYADPVSAQHAKLSLDGQNIYNACCTLRIDFSKLTSLNVKYNNDKSRDYTRPDLPSGDSQPSLDQTMAAAFGAPGIISASPYAGAGFPPTFAIPQAAGLSVPNVHGALAPLAIPSAAAAAAAAGRIAIPGLAGAGNSVLLVSNL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 polypyrimidine tract binding protein 1
别名 3D6D8, HNRNP I, HNRNPI, HNRPI, pPTB
计算分子量 60 kDa
观测分子量 60 kDa
GenBank蛋白编号BC013694
基因名称 PTBP1
Gene ID (NCBI) 5725
RRIDAB_2882692
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP26599
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Polypyrimidine tract-binding protein (PTBP1), also known as heterogeneous nuclear ribonucleoprotein type I (hnRNP I), is a nuclear protein that binds pre-mRNAs in specific regions of the hnRNA-protein complexes sensitive to micrococcal nuclease. It involves in pre-mRNA splicing and the regulation of alternative splicing events. PTBP1 promotes RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA, and promotes the binding of U2 snRNP to pre-mRNA. Besides, it can cooperate with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA, and represses the splicing of MAPT/Tau exon 10.

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