• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

PTBP1 Monoclonal antibody

PTBP1 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3D6D8

Cat No : 67462-1-Ig

Print datasheet

Synonyms

HNRNP I, HNRNPI, HNRPI, pPTB, PTB, PTB 1, PTB T, PTB2, PTB3, PTB4, PTBP1

验证数据展示

  • WB analysis using 67462-1-Ig

    WB analysis using 67462-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67462-1-Ig (PTBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 67462-1-Ig

    IHC staining of human colon cancer using 67462-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67462-1-Ig (PTBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PTBP1 antibody included
  • IF Staining of A375 using 67462-1-Ig

    IF Staining of A375 using 67462-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using 67462-1-Ig (PTBP1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis of HeLa using 67462-1-Ig

    WB analysis of HeLa using 67462-1-Ig

    WB result of PTBP1 antibody (67462-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTBP1 transfected HeLa cells.

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, LNCaP cells, HEK-293 cells. K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman colon cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA375 cells
Planning an IHC experiment? We recommend our IHCeasy PTBP1 Ready-To-Use IHC Kit. PTBP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67462-1-Ig targets PTBP1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, mouse, rat
Cited Reactivitymouse
Immunogen PTBP1 fusion protein Ag28404 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name polypyrimidine tract binding protein 1
Synonyms HNRNP I, HNRNPI, HNRPI, pPTB, PTB, PTB 1, PTB T, PTB2, PTB3, PTB4, PTBP1
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC013694
Gene Symbol PTBP1
Gene ID (NCBI) 5725
RRIDAB_2882692
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP26599
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Polypyrimidine tract-binding protein (PTBP1), also known as heterogeneous nuclear ribonucleoprotein type I (hnRNP I), is a nuclear protein that binds pre-mRNAs in specific regions of the hnRNA-protein complexes sensitive to micrococcal nuclease. It involves in pre-mRNA splicing and the regulation of alternative splicing events. PTBP1 promotes RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA, and promotes the binding of U2 snRNP to pre-mRNA. Besides, it can cooperate with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA, and represses the splicing of MAPT/Tau exon 10.

实验方案

Product Specific Protocols
WB protocol for PTBP1 antibody 67462-1-IgDownload protocol
IHC protocol for PTBP1 antibody 67462-1-IgDownload protocol
IF protocol for PTBP1 antibody 67462-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Toxicol Sci

Propofol improves ischemia reperfusion-induced liver fibrosis by regulating lncRNA HOXA11-AS

Authors - Jia Luo
  • KD Validated
View Article