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CoraLite® Plus 488-conjugated PSMB2 Monoclonal antibody

PSMB2 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3C5C5

Cat No : CL488-68180

Print datasheet

Synonyms

HC7 I, Macropain subunit C7 I, Proteasome component C7 I, Proteasome subunit beta type 2, PSMB2

验证数据展示

  • IF Staining of HepG2 using CL488-68180

    IF Staining of HepG2 using CL488-68180

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 PSMB2 antibody (CL488-68180, Clone: 3C5C5 ) at dilution of 1:200.

  • IF Staining of PC-3 using CL488-68180

    IF Staining of PC-3 using CL488-68180

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using CoraLite® Plus 488 PSMB2 antibody (CL488-68180, Clone: 3C5C5 ) at dilution of 1:200.

  • FC experiment of Jurkat using CL488-68180

    FC experiment of Jurkat using CL488-68180

    1X10^6 Jurkat cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human PSMB2 (CL488-68180, Clone:3C5C5) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHepG2 cells, PC-3 cells
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy PSMB2 Ready-To-Use IHC Kit. PSMB2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68180-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68180 targets PSMB2 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, mouse, rat
Immunogen PSMB2 fusion protein Ag7308 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name proteasome (prosome, macropain) subunit, beta type, 2
Synonyms HC7 I, Macropain subunit C7 I, Proteasome component C7 I, Proteasome subunit beta type 2, PSMB2
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC000268
Gene Symbol PSMB2
Gene ID (NCBI) 5690
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49721
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMB2(proteasome subunit beta type-2) is also named as macropain subunit C7-I, proteasome component C7-I, multicatalytic endopeptidase complex subunit C7-I and belongs to the peptidase T1B family. It is involved in an ATP/ubiquitin-dependent non-lysosomal proteolytic pathway. The up-regulation of PSMB2 may indicate the activated neuronal defensive mechanism in VAD(vitamin A depletion) brain regions, which may underlie the VAD-related psychosis behavior(PMID:21190828).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PSMB2 antibody CL488-68180Download protocol
FC protocol for CL Plus 488 PSMB2 antibody CL488-68180Download protocol
Standard Protocols
Click here to view our Standard Protocols