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  • Featured Product
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PSMB2 Monoclonal antibody

PSMB2 Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3C5C5

Cat No : 68180-1-Ig

Print datasheet

Synonyms

HC7 I, Macropain subunit C7 I, Proteasome component C7 I, Proteasome subunit beta type 2, PSMB2

验证数据展示

  • WB analysis using 68180-1-Ig

    WB analysis using 68180-1-Ig

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 68180-1-Ig (PSMB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68180-1-Ig

    WB analysis using 68180-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68180-1-Ig (PSMB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis of HeLa using 68180-1-Ig

    WB analysis of HeLa using 68180-1-Ig

    WB result of PSMB2 antibody (68180-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMB2 transfected HeLa cells.

  • IF Staining of HCT 116 using 68180-1-Ig

    IF Staining of HCT 116 using 68180-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using PSMB2 antibody (68180-1-Ig, Clone: 3C5C5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of HepG2 using 68180-1-Ig

    IF Staining of HepG2 using 68180-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSMB2 antibody (68180-1-Ig, Clone: 3C5C5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of PC-3 using 68180-1-Ig

    IF Staining of PC-3 using 68180-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using PSMB2 antibody (68180-1-Ig, Clone: 3C5C5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inMCF-7 cells, SKOV-3 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF detected inHepG2 cells, HCT 116 cells, PC-3 cells
Planning an IHC experiment? We recommend our IHCeasy PSMB2 Ready-To-Use IHC Kit. PSMB2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68180-1-Ig targets PSMB2 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen PSMB2 fusion protein Ag7308 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name proteasome (prosome, macropain) subunit, beta type, 2
Synonyms HC7 I, Macropain subunit C7 I, Proteasome component C7 I, Proteasome subunit beta type 2, PSMB2
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC000268
Gene Symbol PSMB2
Gene ID (NCBI) 5690
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49721
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMB2(proteasome subunit beta type-2) is also named as macropain subunit C7-I, proteasome component C7-I, multicatalytic endopeptidase complex subunit C7-I and belongs to the peptidase T1B family. It is involved in an ATP/ubiquitin-dependent non-lysosomal proteolytic pathway. The up-regulation of PSMB2 may indicate the activated neuronal defensive mechanism in VAD(vitamin A depletion) brain regions, which may underlie the VAD-related psychosis behavior(PMID:21190828).

实验方案

Product Specific Protocols
WB protocol for PSMB2 antibody 68180-1-IgDownload protocol
IF protocol for PSMB2 antibody 68180-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols