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验证数据展示

  • WB analysis of A549 using 11887-1-AP (same clone as 11887-1-PBS)

    WB analysis of A549 using 11887-1-AP (same clone as 11887-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 11887-1-AP (same clone as 11887-1-PBS)

    IHC staining of human lymphoma using 11887-1-AP (same clone as 11887-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMA3 antibody included
  • IHC staining of human lymphoma using 11887-1-AP (same clone as 11887-1-PBS)

    IHC staining of human lymphoma using 11887-1-AP (same clone as 11887-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMA3 antibody included
  • WB analysis of human lung using 11887-1-AP (same clone as 11887-1-PBS)

    WB analysis of human lung using 11887-1-AP (same clone as 11887-1-PBS)

    human lung tissue were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

  • WB analysis of PC-12 using 11887-1-AP (same clone as 11887-1-PBS)

    WB analysis of PC-12 using 11887-1-AP (same clone as 11887-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 11887-1-AP (same clone as 11887-1-PBS)

    IHC staining of human liver cancer using 11887-1-AP (same clone as 11887-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMA3 antibody included
  • IHC staining of human liver cancer using 11887-1-AP (same clone as 11887-1-PBS)

    IHC staining of human liver cancer using 11887-1-AP (same clone as 11887-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMA3 antibody included
  • IF Staining of HeLa using 11887-1-AP (same clone as 11887-1-PBS)

    IF Staining of HeLa using 11887-1-AP (same clone as 11887-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMA3 antibody (11887-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

  • WB analysis using 11887-1-AP (same clone as 11887-1-PBS)

    WB analysis using 11887-1-AP (same clone as 11887-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 11887-1-AP (same clone as 11887-1-PBS)

    IHC staining of mouse brain using 11887-1-AP (same clone as 11887-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMA3 antibody included

PSMA3 Polyclonal antibody, PBS Only

PSMA3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11887-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, monkey

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

PSC8, Proteasome subunit alpha type-3, HC8

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PSMA3 Ready-To-Use IHC Kit. PSMA3 primary antibody included.

产品信息

11887-1-PBS targets PSMA3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, monkey
免疫原

CatNo: Ag2495

Product name: Recombinant human PSMA3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-255 aa of BC029402

Sequence: MSSIGTGYDLSASTFSPDGRVFQVEYAMKAVENSSTAIGIRCKDGVVFGVEKLVLSKLYEEGSNKRLFNVDRHVGMAVAGLLADARSLADMAREEASNFRSNFGYNIPLKHLADRVAMYVHAYTLYSAVRPFGCSFMLGSYSVNDGAQLYMIDPSGVSYGYWGCAIGKARQAAKTEIEKLQMKEMTCRDIVKEVAKIIYIVHDEVKDKAFELELSWVGELTNGRHEIVPKDIREEAEKYAKESLKEEDESDDDNM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, alpha type, 3
别名 PSC8, Proteasome subunit alpha type-3, HC8
计算分子量 255 aa, 28 kDa
观测分子量28 kDa
GenBank蛋白编号BC029402
基因名称 PSMA3
Gene ID (NCBI) 5684
RRIDAB_2171420
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP25788
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PSMA3(Proteasome subunit alpha type-3) is also named as HC8, PSC8 and belongs to the peptidase T1A family. The proteasome is a multicatalytic protease complex that catalyzes an energy-dependent, extralysosomal proteolytic pathway responsible for selective elimination of proteins with aberrant structures and naturally occurring short-lived proteins related to metabolic regulation and cell cycle progression. It has 2 isoforms produced by alternative splicing.

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