PSMA3 Polyclonal antibody
PSMA3 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, monkey
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, PC-12 cells, human lung tissue, COS-7 cells, RAW 264.7 cells, mouse spleen tissue, rat brain tissue, HeLa cells, NIH/3T3 cells, mouse liver tissue |
| Positive IHC detected in | human lymphoma tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PSMA3 Ready-To-Use IHC Kit. PSMA3 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11887-1-AP targets PSMA3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
| Tested Applications | IF/ICC, IHC, WB, ELISA |
| Cited Applications | WB, IF, ELISA |
| Tested Reactivity | human, mouse, rat, monkey |
| Cited Reactivity | human, mouse |
| Immunogen | PSMA3 fusion protein Ag2495 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | proteasome (prosome, macropain) subunit, alpha type, 3 |
| Synonyms | 20S proteasome, HC8, Macropain subunit C8, Proteasome component C8, PSC3, PSC8, PSMA3 |
| Calculated Molecular Weight | 255 aa, 28 kDa |
| Observed Molecular Weight | 28 kDa |
| GenBank Accession Number | BC029402 |
| Gene Symbol | PSMA3 |
| Gene ID (NCBI) | 5684 |
| RRID | AB_2171420 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P25788 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PSMA3(Proteasome subunit alpha type-3) is also named as HC8, PSC8 and belongs to the peptidase T1A family. The proteasome is a multicatalytic protease complex that catalyzes an energy-dependent, extralysosomal proteolytic pathway responsible for selective elimination of proteins with aberrant structures and naturally occurring short-lived proteins related to metabolic regulation and cell cycle progression. It has 2 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PSMA3 antibody 11887-1-AP | Download protocol |
| IHC protocol for PSMA3 antibody 11887-1-AP | Download protocol |
| IF protocol for PSMA3 antibody 11887-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Free Radic Biol Med Identification of an unstable 4-hydroxynoneal modification on the 20S proteasome subunit α7 by recombinant antibody technology. | ||
Clin Transl Med FBXW24 controls female meiotic prophase progression by regulating SYCP3 ubiquitination. | ||
Cell Mol Life Sci The insulin-degrading enzyme is an allosteric modulator of the 20S proteasome and a potential competitor of the 19S. | ||
ChemMedChem Pyrazolones Activate the Proteasome by Gating Mechanisms and Protect Neuronal Cells from β-Amyloid Toxicity. | ||
Research (Wash D C) SERBP1 Promotes Stress Granule Clearance by Regulating 26S Proteasome Activity and G3BP1 Ubiquitination and Protects Male Germ Cells from Thermostimuli Damage | ||