验证数据展示

WB analysis of A549 using 11887-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC staining of human lymphoma using 11887-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PSMA3 antibody included
IHC staining of human lymphoma using 11887-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PSMA3 antibody included
WB analysis of human lung using 11887-1-AP
human lung tissue were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of PC-12 using 11887-1-AP
PC-12 cells were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 11887-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PSMA3 antibody included
IHC staining of human liver cancer using 11887-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PSMA3 antibody included
IF Staining of HeLa using 11887-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMA3 antibody (11887-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
WB analysis using 11887-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 11887-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PSMA3 antibody included

PSMA3 Polyclonal antibody

PSMA3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11887-1-AP

产品说明书

引用文献(7)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, monkey

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

PSC8, Proteasome subunit alpha type-3, HC8

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (7)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	A549 cells, PC-12 cells, human lung tissue, COS-7 cells, RAW 264.7 cells, mouse spleen tissue, rat brain tissue, HeLa cells, NIH/3T3 cells, mouse liver tissue
Positive IHC detected in	human lymphoma tissue, human liver cancer tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy PSMA3 Ready-To-Use IHC Kit. PSMA3 primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:200-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 4 publications below
IF	See 1 publications below
ELISA	See 1 publications below

产品信息

11887-1-AP targets PSMA3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

经测试应用	WB, IHC, IF/ICC, ELISA Application Description
文献引用应用	WB, IF, ELISA
经测试反应性	human, mouse, rat, monkey
文献引用反应性	human, mouse
免疫原	CatNo: Ag2495 Product name: Recombinant human PSMA3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-255 aa of BC029402 Sequence: MSSIGTGYDLSASTFSPDGRVFQVEYAMKAVENSSTAIGIRCKDGVVFGVEKLVLSKLYEEGSNKRLFNVDRHVGMAVAGLLADARSLADMAREEASNFRSNFGYNIPLKHLADRVAMYVHAYTLYSAVRPFGCSFMLGSYSVNDGAQLYMIDPSGVSYGYWGCAIGKARQAAKTEIEKLQMKEMTCRDIVKEVAKIIYIVHDEVKDKAFELELSWVGELTNGRHEIVPKDIREEAEKYAKESLKEEDESDDDNM 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	proteasome (prosome, macropain) subunit, alpha type, 3
别名	PSC8, Proteasome subunit alpha type-3, HC8
计算分子量	255 aa, 28 kDa
观测分子量	28 kDa
GenBank蛋白编号	BC029402
基因名称	PSMA3
Gene ID (NCBI)	5684
RRID	AB_2171420
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P25788
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

PSMA3(Proteasome subunit alpha type-3) is also named as HC8, PSC8 and belongs to the peptidase T1A family. The proteasome is a multicatalytic protease complex that catalyzes an energy-dependent, extralysosomal proteolytic pathway responsible for selective elimination of proteins with aberrant structures and naturally occurring short-lived proteins related to metabolic regulation and cell cycle progression. It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for PSMA3 antibody 11887-1-AP	Download protocol
IHC protocol for PSMA3 antibody 11887-1-AP	Download protocol
IF protocol for PSMA3 antibody 11887-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	ELISA	Free Radic Biol Med Identification of an unstable 4-hydroxynoneal modification on the 20S proteasome subunit α7 by recombinant antibody technology. Authors - Jesper Just View Article
mouse	IF	Clin Transl Med FBXW24 controls female meiotic prophase progression by regulating SYCP3 ubiquitination. Authors - Yang Wang View Article
human	WB	JCI Insight Endothelial extracellular vesicle miR-423-5p regulates microvascular homeostasis and renal function after ischemia-reperfusion injury Authors - Francis Migneault View Article
		Cell Mol Life Sci The insulin-degrading enzyme is an allosteric modulator of the 20S proteasome and a potential competitor of the 19S. Authors - Diego Sbardella View Article
human	WB	ChemMedChem Pyrazolones Activate the Proteasome by Gating Mechanisms and Protect Neuronal Cells from β-Amyloid Toxicity. Authors - Anna Maria Santoro View Article
human	WB	Research (Wash D C) SERBP1 Promotes Stress Granule Clearance by Regulating 26S Proteasome Activity and G3BP1 Ubiquitination and Protects Male Germ Cells from Thermostimuli Damage Authors - Fengli Wang View Article

验证数据展示

WB analysis of A549 using 11887-1-AP

IHC staining of human lymphoma using 11887-1-AP

IHC staining of human lymphoma using 11887-1-AP

WB analysis of human lung using 11887-1-AP

WB analysis of PC-12 using 11887-1-AP

IHC staining of human liver cancer using 11887-1-AP

IHC staining of human liver cancer using 11887-1-AP

IF Staining of HeLa using 11887-1-AP

WB analysis using 11887-1-AP

IHC staining of mouse brain using 11887-1-AP

PSMA3 Polyclonal antibody

PSMA3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Identification of an unstable 4-hydroxynoneal modification on the 20S proteasome subunit α7 by recombinant antibody technology.

FBXW24 controls female meiotic prophase progression by regulating SYCP3 ubiquitination.

Endothelial extracellular vesicle miR-423-5p regulates microvascular homeostasis and renal function after ischemia-reperfusion injury

The insulin-degrading enzyme is an allosteric modulator of the 20S proteasome and a potential competitor of the 19S.

Pyrazolones Activate the Proteasome by Gating Mechanisms and Protect Neuronal Cells from β-Amyloid Toxicity.

SERBP1 Promotes Stress Granule Clearance by Regulating 26S Proteasome Activity and G3BP1 Ubiquitination and Protects Male Germ Cells from Thermostimuli Damage