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验证数据展示

  • WB analysis using 68240-1-Ig

    WB analysis using 68240-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68240-1-Ig (PSMA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 68240-1-Ig

    IHC staining of human cervical cancer using 68240-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68240-1-Ig (PSMA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMA2 antibody included
  • IHC staining of human cervical cancer using 68240-1-Ig

    IHC staining of human cervical cancer using 68240-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68240-1-Ig (PSMA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMA2 antibody included

PSMA2 Monoclonal antibody

PSMA2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68240-1-Ig

产品说明书

CloneNo. 2H4A10

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, pig, rat

应用

WB, IHC, ELISA

别名

HC3, Macropain subunit C3, MU, PMSA2, Proteasome component C3, PSC2, PSC3, PSMA2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inU2OS cells, A431 cells, HEK-293 cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, pig heart tissue, rat heart tissue, mouse heart tissue
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PSMA2 Ready-To-Use IHC Kit. PSMA2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68240-1-Ig targets PSMA2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, pig, rat
免疫原

CatNo: Ag5837

Product name: Recombinant human PSMA2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-234 aa of BC047697

Sequence: MAERGYSFSLTTFSPSGKLVQIEYALAAVAGGAPSVGIKAANGVVLATEKKQKSILYDERSVHKVEPITKHIGLVYSGMGPDYRVLVHRARKLAQQYYLVYQEPIPTAQLVQRVASVMQEYTQSGGVRPFGVSLLICGWNEGRPYLFQSDPSGAYFAWKATAMGKNYVNGKTFLEKRYNEDLELEDAIHTAILTLKESFEGQMTEDNIEVGICNEAGFRRLTPTEVKDYLAAIA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, alpha type, 2
别名 HC3, Macropain subunit C3, MU, PMSA2, Proteasome component C3, PSC2, PSC3, PSMA2
计算分子量 26 kDa
观测分子量 26 kDa
GenBank蛋白编号BC047697
基因名称 PSMA2
Gene ID (NCBI) 5683
RRIDAB_2935327
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP25787
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMA2 (Proteasome subunit alpha type-2) is the essential subunit that contributes to the complete assembly of 20S proteasome complex, involved in the proteolytic degradation of most intracellular proteins. It is required in spermatogenesis and generation of a subset of MHC class I-presented antigenic peptides(PMID: 34711951, PMID: 27342858). Dysregulation of PSMA2 degradation leads to tumorigenesis including cervical cancer (PMID:33684886).

实验方案

Product Specific Protocols
WB protocol for PSMA2 antibody 68240-1-IgDownload protocol
IHC protocol for PSMA2 antibody 68240-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols