验证数据展示
经过测试的应用
| Positive WB detected in | U2OS cells, A431 cells, HEK-293 cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, pig heart tissue, rat heart tissue, mouse heart tissue |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68240-1-Ig targets PSMA2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | Human, mouse, pig, rat |
| 免疫原 |
CatNo: Ag5837 Product name: Recombinant human PSMA2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-234 aa of BC047697 Sequence: MAERGYSFSLTTFSPSGKLVQIEYALAAVAGGAPSVGIKAANGVVLATEKKQKSILYDERSVHKVEPITKHIGLVYSGMGPDYRVLVHRARKLAQQYYLVYQEPIPTAQLVQRVASVMQEYTQSGGVRPFGVSLLICGWNEGRPYLFQSDPSGAYFAWKATAMGKNYVNGKTFLEKRYNEDLELEDAIHTAILTLKESFEGQMTEDNIEVGICNEAGFRRLTPTEVKDYLAAIA 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | proteasome (prosome, macropain) subunit, alpha type, 2 |
| 别名 | HC3, Macropain subunit C3, MU, PMSA2, Proteasome component C3, PSC2, PSC3, PSMA2 |
| 计算分子量 | 26 kDa |
| 观测分子量 | 26 kDa |
| GenBank蛋白编号 | BC047697 |
| 基因名称 | PSMA2 |
| Gene ID (NCBI) | 5683 |
| RRID | AB_2935327 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P25787 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PSMA2 (Proteasome subunit alpha type-2) is the essential subunit that contributes to the complete assembly of 20S proteasome complex, involved in the proteolytic degradation of most intracellular proteins. It is required in spermatogenesis and generation of a subset of MHC class I-presented antigenic peptides(PMID: 34711951, PMID: 27342858). Dysregulation of PSMA2 degradation leads to tumorigenesis including cervical cancer (PMID:33684886).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PSMA2 antibody 68240-1-Ig | Download protocol |
| WB protocol for PSMA2 antibody 68240-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
