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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 85395-1-RR (same clone as 85395-1-PBS)

    WB analysis using 85395-1-RR (same clone as 85395-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85395-1-RR (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85395-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 85395-1-RR (same clone as 85395-1-PBS)

    IHC staining of human liver cancer using 85395-1-RR (same clone as 85395-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 85395-1-RR (PSIP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85395-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSIP1 antibody included
  • IHC staining of human liver cancer using 85395-1-RR (same clone as 85395-1-PBS)

    IHC staining of human liver cancer using 85395-1-RR (same clone as 85395-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 85395-1-RR (PSIP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85395-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSIP1 antibody included
  • IF Staining of HepG2 using 85395-1-RR (same clone as 85395-1-PBS)

    IF Staining of HepG2 using 85395-1-RR (same clone as 85395-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSIP1 antibody (85395-1-RR, Clone: 242841C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85395-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85395-1-RR

    Affinity and Kinetic Characterization of 85395-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85395-1-RR against Human PSIP1 were performed. The affinity constant is below 1 pM.

PSIP1 Recombinant antibody, PBS Only

PSIP1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 85395-1-PBS

产品说明书

CloneNo. 242841C5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

242841C5, CLL associated antigen KW 7, CLL-associated antigen KW-7, DFS70, LEDGF

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PSIP1 Ready-To-Use IHC Kit. PSIP1 primary antibody included.

产品信息

85395-1-PBS targets PSIP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PSIP1 fusion protein Ag21886 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 PC4 and SFRS1 interacting protein 1
别名 242841C5, CLL associated antigen KW 7, CLL-associated antigen KW-7, DFS70, LEDGF
计算分子量 530 aa, 60 kDa
观测分子量75 kDa
GenBank蛋白编号BC064135
基因名称 PSIP1
Gene ID (NCBI) 11168
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75475
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PSIP1, also known as PC4 and SRSF1 Interacting Protein 1 or Lens Epithelium-Derived Growth Factor (LEDGF), is a transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. It also plays a role in lens epithelial cell gene regulation and stress responses. It is a chromatin-associated protein with histone chaperone activity, involved in transcriptional elongation. PSIP1 may act as an adapter to coordinate pre-mRNA splicing. PSIP1 encodes two protein isoforms with molecular masses of 52 and 75 kDa. p52 and p75 were identified as interacting with transcription factor PC4 and shown to act as transcriptional coactivators.