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验证数据展示

  • FC experiment of A431 using 32522-1-AP (same clone as 32522-1-PBS)

    FC experiment of A431 using 32522-1-AP (same clone as 32522-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Prss21 Polyclonal Antibody (32522-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 32522-1-PBS in a different storage buffer formulation.

  • WB analysis using 32522-1-AP (same clone as 32522-1-PBS)

    WB analysis using 32522-1-AP (same clone as 32522-1-PBS)

    Mouse testis lysates were subjected to SDS PAGE followed by western blot with 32522-1-AP (PRSS21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32522-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 32522-1-AP (same clone as 32522-1-PBS)

    IHC staining of mouse testis using 32522-1-AP (same clone as 32522-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 32522-1-AP (PRSS21 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32522-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 32522-1-AP (same clone as 32522-1-PBS)

    IHC staining of mouse testis using 32522-1-AP (same clone as 32522-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 32522-1-AP (PRSS21 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32522-1-PBS in a different storage buffer formulation.

PRSS21 Polyclonal antibody, PBS Only

PRSS21 Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 32522-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

EC:3.4.21.-, Eosinophil serine protease 1, ESP1, ESP-1, Serine protease 21

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

32522-1-PBS targets PRSS21 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag38156

Product name: Recombinant human PRSS21 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-314 aa of BC074999

Sequence: MGARGALLLALLLARAGLRKPESQEAAPLSGPCGRRVITSRIVGGEDAELGRWPWQGSLRLWDSHVCGVSLLSHRWALTAAHCFETYSDLSDPSGWMVQFGQLTSMPSFWSLQAYYTRYFVSNIYLSPRYLGNSPYDIALVKLSAPVTYTKHIQPICLQASTFEFENRTDCWVTGWGYIKEDEALPSPHTLQEVQVAIINNSMCNHLFLKYSFRKDIFGDMVCAGNAQGGKDACFGDSGGPLACNKNGLWYQIGVVSWGVGCGRPNRPGVYTNISHHFEWIQKLMAQSGMSQPDPSWPLLFFPLLWALPLLGPV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protease, serine, 21 (testisin)
别名 EC:3.4.21.-, Eosinophil serine protease 1, ESP1, ESP-1, Serine protease 21
计算分子量 314 aa, 35 kDa
GenBank蛋白编号BC074999
基因名称 PRSS21
Gene ID (NCBI) 10942
RRIDAB_3742642
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6M0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PRSS21 (Serine Protease 21), a trypsin family member, may be active on peptide linkages involving the carboxyl group of lysine or arginine. In terms of disease association, PRSS21 is implicated in various cancers, such as gastric cancer, testicular cancer, and ovarian cancer.

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