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验证数据展示

  • WB analysis using 67751-1-Ig (same clone as 67751-1-PBS)

    WB analysis using 67751-1-Ig (same clone as 67751-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67751-1-Ig (PRKAR2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67751-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67751-1-Ig (same clone as 67751-1-PBS)

    IHC staining of human colon cancer using 67751-1-Ig (same clone as 67751-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67751-1-Ig (PRKAR2A antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67751-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKAR2A antibody included
  • IHC staining of human colon cancer using 67751-1-Ig (same clone as 67751-1-PBS)

    IHC staining of human colon cancer using 67751-1-Ig (same clone as 67751-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67751-1-Ig (PRKAR2A antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67751-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKAR2A antibody included
  • IF Staining of HeLa using 67751-1-Ig (same clone as 67751-1-PBS)

    IF Staining of HeLa using 67751-1-Ig (same clone as 67751-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PRKAR2A antibody (67751-1-Ig, Clone: 1B2B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67751-1-PBS in a different storage buffer formulation.

PRKAR2A Monoclonal antibody, PBS Only

PRKAR2A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67751-1-PBS

产品说明书

CloneNo. 1B2B3

宿主/亚型

Mouse / IgG1

种属反应性

Human, rat, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

PKR2, PRKAR2, PRKAR2A

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PRKAR2A Ready-To-Use IHC Kit. PRKAR2A primary antibody included.

产品信息

67751-1-PBS targets PRKAR2A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, rat, mouse
免疫原

CatNo: Ag29893

Product name: Recombinant human PRKAR2A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 74-382 aa of BC002763

Sequence: AKGDSESEEDEDLEVPVPSRFNRRVSVCAETYNPDEEEEDTDPRVIHPKTDEQRCRLQEACKDILLFKNLDQEQLSQVLDAMFERIVKADEHVIDQGDDGDNFYVIERGTYDILVTKDNQTRSVGQYDNRGSFGELALMYNTPRAATIVATSEGSLWGLDRVTFRRIIVKNNAKKRKMFESFIESVPLLKSLEVSERMKIVDVIGEKIYKDGERIITQTKSNKDGGNQEVEIARCHKGQYFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKMFGSSVDLGNLGQ

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein kinase, cAMP-dependent, regulatory, type II, alpha
别名 PKR2, PRKAR2, PRKAR2A
计算分子量 43 kDa
观测分子量50 kDa
GenBank蛋白编号BC002763
基因名称 PRKAR2A
Gene ID (NCBI) 5576
RRIDAB_2918520
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP13861
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The effects of cAMP in most tissues and cell types are mainly modulated via protein kinase A, a heterotetrameric protein complex consisting of two regulatory and two catalytic subunits. The regulatory subunit of cAMP-dependent protein kinase (PRKAR2A) is one of the regulatory subunits and the gene is located on chromosome region 3p21.3-p21.2. The expression of PRKAR2A is tightly regulated during spermatogenesis, a significant increase in expression of this gene was also found in the human myometrium during pregnancy.

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