• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of HepG2 using CL647-10703

    IF Staining of HepG2 using CL647-10703

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 647 PRDX4 antibody (CL647-10703) at dilution of 1:200.

  • FC experiment of HepG2 using CL647-10703

    FC experiment of HepG2 using CL647-10703

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human PRDX4 (CL647-10703) (red), or 0.8 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated PRDX4 Polyclonal antibody

PRDX4 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL647-10703

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

Antioxidant enzyme AOE372, AOE37 2, AOE37-2, EC:1.11.1.24, peroxiredoxin 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PRDX4 Ready-To-Use IHC Kit. PRDX4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10703-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-10703 targets PRDX4 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 PRDX4 fusion protein Ag1177 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peroxiredoxin 4
别名 Antioxidant enzyme AOE372, AOE37 2, AOE37-2, EC:1.11.1.24, peroxiredoxin 4
计算分子量 31 kDa
观测分子量 30 kDa
GenBank蛋白编号BC007107
基因名称 PRDX4
Gene ID (NCBI) 10549
RRIDAB_2934831
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13162
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRDX4 (Peroxiredoxin-4) is also named as AOE37-2, Prx-IV and belongs to the AhpC/TSA family. PRDX4 is associated with acrosome formation during rat spermatogenesis and has a protective role in the male reproductive tract, because the phenotype of mice lacking this isoform includes testicular atrophy and increased sperm DNA damage (PMID:20864641). It is initially synthesized as a membrane-binding 31-kDa protein and processed into a 27-kDa secretory form and is discarded with the residual bodies (PMID:19208552). PRDX4 is a pentamer of dimers (PMID:23025503). This antibody is specific to PRDX4.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 PRDX4 antibody CL647-10703Download protocol
FC protocol for CL Plus 647 PRDX4 antibody CL647-10703Download protocol
Standard Protocols
Click here to view our Standard Protocols