验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PPP3CA Ready-To-Use IHC Kit. PPP3CA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13422-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-13422 targets PPP3CA in FC (Intra) applications and shows reactivity with human, mouse samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | PPP3CA fusion protein Ag4203 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform |
| 别名 | CALN, CALNA, CALNA1, CAM PRP catalytic subunit, CCN1, CNA, CNA1, PP2BA, PPP2B, PPP3CA |
| 计算分子量 | 59 kDa |
| 观测分子量 | 59 kDa |
| GenBank蛋白编号 | BC025714 |
| 基因名称 | PPP3CA |
| Gene ID (NCBI) | 5530 |
| RRID | AB_2934886 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q08209 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PPP3CA, also called calcineurin Aα, is the only serine/threonine protein phosphatase under the control of Ca2+/calmodulin, and plays a critical role in the coupling of Ca2+ signals to cellular responses. Ca2+ signaling plays a central role in hypertrophic growth of cardiac and skeletal muscle in response to mechanical load and a variety of signals. Therefore, PPP3CA plays an important role in muscle differentiation, especially in muscle fiber type conversion. It is also involved in osteoclast regulation, regulating bone formation through an effect on osteoblast differentiation.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 PPP3CA antibody CL647-13422 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |