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验证数据展示

  • WB analysis of mouse heart using 27285-1-AP

    WB analysis of mouse heart using 27285-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 27285-1-AP (PPP1R3B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 27285-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 27285-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27285-1-AP (PPP1R3B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 27285-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 27285-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27285-1-AP (PPP1R3B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PPP1R3B Polyclonal antibody

PPP1R3B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27285-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

PTG, PPP1R4, PP1 subunit R4, Hepatic glycogen-targeting protein phosphatase 1 regulatory subunit GL

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, MDA-MB-453s cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27285-1-AP targets PPP1R3B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 PPP1R3B fusion protein Ag26025 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein phosphatase 1, regulatory (inhibitor) subunit 3B
别名 PTG, PPP1R4, PP1 subunit R4, Hepatic glycogen-targeting protein phosphatase 1 regulatory subunit GL
计算分子量 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC043388
基因名称 PPP1R3B
Gene ID (NCBI) 79660
RRIDAB_2880829
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86XI6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP1R3B, also known as Protein Phosphatase 1, Regulatory Subunit 3B, is a protein encoded by the PPP1R3B gene in humans. It is a regulatory subunit of the serine/threonine-specific protein phosphatase 1 (PP1), an enzyme that plays a crucial role in the control of a wide range of cellular processes by dephosphorylating target proteins.

实验方案

Product Specific Protocols
WB protocol for PPP1R3B antibody 27285-1-APDownload protocol
IHC protocol for PPP1R3B antibody 27285-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols