验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HeLa cells, HepG2 cells, MCF- cells, Jurkat cells, mouse brain tissue, rat brain tissue |
| Positive IF/ICC detected in | MCF-7 cells, A549 cells |
| Positive FC (Intra) detected in | Jurkat cells |
Planning an IHC experiment? We recommend our IHCeasy PPP1CB Ready-To-Use IHC Kit. PPP1CB primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84938-5-RR targets PPP1CB in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, FC (Intra), ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | PPP1CB fusion protein Ag0183 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | protein phosphatase 1, catalytic subunit, beta isoform |
| 别名 | PPP1CD, PP-1B, PP1 Beta, PP 1B, EC:3.1.3.16 |
| 计算分子量 | 37 kDa |
| 观测分子量 | 35-37 kDa |
| GenBank蛋白编号 | BC002697 |
| 基因名称 | PPP1CB |
| Gene ID (NCBI) | 5500 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P62140 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein phosphatase 1 (PP1) is the main eukaryotic protein serine/threonine phosphatase that regulates an enormous variety of cellular processes including protein-protein interactions, gene transcription, cell-cycle progression and apoptosis. It is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. The catalytic subunit of PP1 (PP1c) can interact with amount of regulatory subunits in vivo that target it to particular subcellular locations and regulate its activity towards specific substrates.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPP1CB antibody 84938-5-RR | Download protocol |
| IF protocol for PPP1CB antibody 84938-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
