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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84938-5-RR (same clone as 84938-5-PBS)

    WB analysis using 84938-5-RR (same clone as 84938-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84938-5-RR (PPP1CB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84938-5-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 84938-5-RR (same clone as 84938-5-PBS)

    FC experiment of Jurkat using 84938-5-RR (same clone as 84938-5-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug PPP1CB Recombinant antibody (84938-5-RR, Clone:242547H7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84938-5-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 84938-5-RR (same clone as 84938-5-PBS)

    IF Staining of MCF-7 using 84938-5-RR (same clone as 84938-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PPP1CB antibody (84938-5-RR, Clone: 242547H7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84938-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84938-5-RR

    Affinity and Kinetic Characterization of 84938-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84938-5-RR against Human PPP1CB were performed. The affinity constant is 2.44 nM.

PPP1CB Recombinant antibody, PBS Only

PPP1CB Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84938-5-PBS

产品说明书

CloneNo. 242547H7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

PPP1CD, PP-1B, PP1 Beta, PP 1B, EC:3.1.3.16

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PPP1CB Ready-To-Use IHC Kit. PPP1CB primary antibody included.

产品信息

84938-5-PBS targets PPP1CB in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PPP1CB fusion protein Ag0183 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein phosphatase 1, catalytic subunit, beta isoform
别名 PPP1CD, PP-1B, PP1 Beta, PP 1B, EC:3.1.3.16
计算分子量 37 kDa
观测分子量 35-37 kDa
GenBank蛋白编号BC002697
基因名称 PPP1CB
Gene ID (NCBI) 5500
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP62140
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protein phosphatase 1 (PP1) is the main eukaryotic protein serine/threonine phosphatase that regulates an enormous variety of cellular processes including protein-protein interactions, gene transcription, cell-cycle progression and apoptosis. It is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. The catalytic subunit of PP1 (PP1c) can interact with amount of regulatory subunits in vivo that target it to particular subcellular locations and regulate its activity towards specific substrates.