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验证数据展示

  • IF Staining of HepG2 using CL488-84870-4

    IF Staining of HepG2 using CL488-84870-4

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 PPIL6 antibody (CL488-84870-4, Clone: 242321G10 ) at dilution of 1:200, CL594-Phalloidin (red).

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CoraLite® Plus 488-conjugated PPIL6 Recombinant monoclonal antibody

PPIL6 Uni-rAb® Recombinant Antibody for IF/ICC
Cat No. CL488-84870-4

产品说明书

CloneNo. 242321G10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

242321G10, Cyclophilin like protein PPIL6, Cyclophilin-like protein PPIL6, PPIase, Probable inactive peptidyl-prolyl cis-trans isomerase-like 6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 84870-4-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-84870-4 targets PPIL6 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原

CatNo: Ag11578

Product name: Recombinant human PPIL6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-311 aa of BC038716

Sequence: MARPQPCGPPHARCGSPSLPERPLQVKVVGLFSCPNFQIAKSAAENLKNNHPSKFEDPILVPLQEFAWHQYLQEKKRELKNETWEYSSSVISFVNGQFLGDALDLQKWAHEVWDIVDIKPSALYDALTEDFSAKFLRDTKHDFVFLDICIDSSPIGRLIFELYCDVCPKTCKNFQVLCTGKAGFSQRGIRLHYKNSIFHRIVQNGWIQGGDIVYGKGDNGESIYGPTFEDENFSVPHNKRGVLGMANKGRHSNGSQFYITLQATPYLDRKFVAFGQLIEGTEVLKQLELVPTQNERPIHMCRITDSGDPYA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 peptidylprolyl isomerase (cyclophilin)-like 6
别名 242321G10, Cyclophilin like protein PPIL6, Cyclophilin-like protein PPIL6, PPIase, Probable inactive peptidyl-prolyl cis-trans isomerase-like 6
计算分子量 311 aa, 35 kDa
GenBank蛋白编号BC038716
基因名称 PPIL6
Gene ID (NCBI) 285755
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8IXY8
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PPIL6 antibody CL488-84870-4Download protocol
Standard Protocols
Click here to view our Standard Protocols