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验证数据展示

  • WB analysis using 29765-1-AP (same clone as 29765-1-PBS)

    WB analysis using 29765-1-AP (same clone as 29765-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29765-1-AP (POLD2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 29765-1-AP (same clone as 29765-1-PBS)

    IHC staining of human colon using 29765-1-AP (same clone as 29765-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29765-1-AP (POLD2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with POLD2 antibody included
  • IF Staining of HeLa using 29765-1-AP (same clone as 29765-1-PBS)

    IF Staining of HeLa using 29765-1-AP (same clone as 29765-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POLD2 antibody (29765-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.

POLD2 Polyclonal antibody, PBS Only

POLD2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29765-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

DNA polymerase delta subunit 2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy POLD2 Ready-To-Use IHC Kit. POLD2 primary antibody included.

产品信息

29765-1-PBS targets POLD2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag31521

Product name: Recombinant human POLD2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 40-195 aa of BC000459

Sequence: RLGERSFSRQYAHIYATRLIQMRPFLENRAQQHWGSGVGVKKLCELQPEEKCCVVGTLFKAMPLQPSILREVSEEHNLLPQPPRSKYIHPDDELVLEDELQRIKLKGTIDVSKLVTGTVLAVFGSVRDDGKFLVEDYCFADLAPQKPAPPLDTDRF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 polymerase (DNA directed), delta 2, regulatory subunit 50kDa
别名 DNA polymerase delta subunit 2
计算分子量 51 kDa
观测分子量48-51 kDa
GenBank蛋白编号BC000459
基因名称 POLD2
Gene ID (NCBI) 5425
RRIDAB_2918344
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49005
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

POLD2, also named as DNA polymerase delta subunit 2, is a 469 amino acid protein, which belongs to the DNA polymerase delta/II small subunit family. The function of the small subunit is not yet clear. POLD2 has an essential function for the small subunit in the heterodimeric core enzyme.

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