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验证数据展示

  • WB analysis using 29765-1-AP

    WB analysis using 29765-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29765-1-AP (POLD2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 29765-1-AP

    IHC staining of human colon using 29765-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29765-1-AP (POLD2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with POLD2 antibody included
  • IF Staining of HeLa using 29765-1-AP

    IF Staining of HeLa using 29765-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POLD2 antibody (29765-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

POLD2 Polyclonal antibody

POLD2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 29765-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

DNA polymerase delta subunit 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, NIH/3T3 cells, HeLa cells, Jurkat cells, SGC-7901 cells, U-251 cells, mouse colon tissue, mouse pancreas tissue, rat colon tissue
Positive IHC detected inhuman colon tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy POLD2 Ready-To-Use IHC Kit. POLD2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29765-1-AP targets POLD2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag31521

Product name: Recombinant human POLD2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 40-195 aa of BC000459

Sequence: RLGERSFSRQYAHIYATRLIQMRPFLENRAQQHWGSGVGVKKLCELQPEEKCCVVGTLFKAMPLQPSILREVSEEHNLLPQPPRSKYIHPDDELVLEDELQRIKLKGTIDVSKLVTGTVLAVFGSVRDDGKFLVEDYCFADLAPQKPAPPLDTDRF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 polymerase (DNA directed), delta 2, regulatory subunit 50kDa
别名 DNA polymerase delta subunit 2
计算分子量 51 kDa
观测分子量48-51 kDa
GenBank蛋白编号BC000459
基因名称 POLD2
Gene ID (NCBI) 5425
RRIDAB_2918344
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49005
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POLD2, also named as DNA polymerase delta subunit 2, is a 469 amino acid protein, which belongs to the DNA polymerase delta/II small subunit family. The function of the small subunit is not yet clear. POLD2 has an essential function for the small subunit in the heterodimeric core enzyme.

实验方案

Product Specific Protocols
WB protocol for POLD2 antibody 29765-1-APDownload protocol
IHC protocol for POLD2 antibody 29765-1-APDownload protocol
IF protocol for POLD2 antibody 29765-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols