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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated POFUT1 Polyclonal antibody

POFUT1 Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra), IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-14929

Print datasheet

Synonyms

FUT12, KIAA0180, O Fuc T, O FucT 1, O FUT, Peptide O fucosyltransferase 1, POFUT1, protein O fucosyltransferase 1

验证数据展示

  • FC experiment of A431 using CL488-14929

    FC experiment of A431 using CL488-14929

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human POFUT1 (CL488-14929) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HUVEC using CL488-14929

    IF Staining of HUVEC using CL488-14929

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CoraLite® Plus 488 POFUT1 antibody (CL488-14929) at dilution of 1:200.

经过测试的应用

Positive IF detected inHUVEC cells
Positive FC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 14929-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-14929 targets POFUT1 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF
Tested Reactivity human, mouse, rat
Immunogen POFUT1 fusion protein Ag6710 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein O-fucosyltransferase 1
Synonyms FUT12, KIAA0180, O Fuc T, O FucT 1, O FUT, Peptide O fucosyltransferase 1, POFUT1, protein O fucosyltransferase 1
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC000582
Gene Symbol POFUT1
Gene ID (NCBI) 23509
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H488
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POFUT1 (protein O-fucosyltransferase 1), also known as GDP-fucose protein O-fucosyltransferase, is an enzyme that catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains and plays a crucial role in Notch signaling.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 POFUT1 antibody CL488-14929Download protocol
FC protocol for CL Plus 488 POFUT1 antibody CL488-14929Download protocol
Standard Protocols
Click here to view our Standard Protocols