验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, HUVEC cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver cancer tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HUVEC cells, A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14929-1-AP targets POFUT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag6710 Product name: Recombinant human POFUT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-182 aa of BC000582 Sequence: MGAAAWARPLSVSFLLLLLPLPGMPAGSWDPAGYLLYCPCMGRFGNQADHFLGSLAFAKLLNRTLAVPPWIEYQHHKPPFTNLHVSYQKYFKLEPLQAYHRVISLEDFMEKLAPTHWPPEKRVAYCFEVAAQRSPDKKTCPMKEGNPFGPFWDQFHVSFNKSELFTGISFSASYREQWSQRR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein O-fucosyltransferase 1 |
| 别名 | EC:2.4.1.221, FUT12, GDP-fucose protein O-fucosyltransferase 1, KIAA0180, O Fuc T |
| 计算分子量 | 44 kDa |
| 观测分子量 | 40-45 kDa |
| GenBank蛋白编号 | BC000582 |
| 基因名称 | POFUT1 |
| Gene ID (NCBI) | 23509 |
| RRID | AB_2166123 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H488 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein O-fucosyltransferase 1 (POFUT1) is an endoplasmic reticulum-resident glycosyltransferase that transfers O-fucose from GDP-fucose to conserved serine or threonine residues within epidermal growth factor-like (EGF) repeats of cell surface and secreted proteins, most notably the Notch receptor family. This modification is essential for Notch signaling: POFUT1-mediated O-fucosylation on Notch EGF repeats directly modulates ligand binding and is required for proper receptor trafficking and canonical signal activation, while also serving as a prerequisite for further glycan elongation by Fringe glycosyltransferases. Beyond Notch, POFUT1 fucosylates other EGF-repeat-containing substrates and functions in an endoplasmic reticulum quality control capacity, stabilizing folded EGF domains. Genetic ablation of Pofut1 in mice is embryonic lethal with phenotypes mirroring global Notch inactivation, and heterozygous mutations in human POFUT1 have been linked to Dowling-Degos disease, underscoring its physiological importance in development and tissue homeostasis.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for POFUT1 antibody 14929-1-AP | Download protocol |
| IHC protocol for POFUT1 antibody 14929-1-AP | Download protocol |
| IP protocol for POFUT1 antibody 14929-1-AP | Download protocol |
| WB protocol for POFUT1 antibody 14929-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8+ T cell fate decisions.
| ||
Nat Commun Defective O-fucosylation of EDIL3 attenuates invasion and vascular remodeling of trophoblasts via LIFR signaling pathway in preeclampsia. | ||
Cell Death Dis poFUT1 promotes uterine angiogenesis and vascular remodeling via enhancing the O-fucosylation on uPA.
| ||
Cell Death Dis LIF upregulates poFUT1 expression and promotes trophoblast cell migration and invasion at the fetal-maternal interface. | ||
Cell Death Dis POFUT1 promotes colorectal cancer development through the activation of Notch1 signaling.
| ||
EBioMedicine PLAGL2 and POFUT1 are regulated by an evolutionarily conserved bidirectional promoter and are collaboratively involved in colorectal cancer by maintaining stemness.
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