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验证数据展示

  • IF Staining of HeLa using 85048-5-RR (same clone as 85048-5-PBS)

    IF Staining of HeLa using 85048-5-RR (same clone as 85048-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PML antibody (85048-5-RR, Clone: 242564C10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85048-5-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 85048-5-RR (same clone as 85048-5-PBS)

    FC experiment of A431 using 85048-5-RR (same clone as 85048-5-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug PML Recombinant antibody (85048-5-RR, Clone:242564C10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 85048-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85048-5-RR

    Affinity and Kinetic Characterization of 85048-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85048-5-RR against Human PML were performed. The affinity constant is below 1 pM.

PML Recombinant antibody, PBS Only

PML Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 85048-5-PBS

产品说明书

CloneNo. 242564C10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Indirect ELISA

别名

242564C10, E3 SUMO-protein ligase PML, EC:2.3.2.-, Protein PML, RING-type E3 SUMO transferase PML

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PML Ready-To-Use IHC Kit. PML primary antibody included.

产品信息

85048-5-PBS targets PML in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 PML fusion protein Ag15377 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 promyelocytic leukemia
别名 242564C10, E3 SUMO-protein ligase PML, EC:2.3.2.-, Protein PML, RING-type E3 SUMO transferase PML
计算分子量 882 aa, 98 kDa
GenBank蛋白编号BC020994
基因名称 PML
Gene ID (NCBI) 5371
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP29590
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PML, also named as MYL, RNF71 and TRIM19, is a member of RBCC/TRIM family of proteins that possess a RING finger domain, B-box, and coiled-coil domain. It functions as tumor suppressor. PML is required for normal, caspase-dependent apoptosis in response to DNA damage, FAS, TNF, or interferons. It plays a role in transcription regulation, DNA damage response, DNA repair and chromatin organization. PML plays a role in processes regulated by retinoic acid, regulation of cell division, terminal differentiation of myeloid precursor cells and differentiation of neural progenitor cells. It regulates PTEN compartmentalization through the inhibition of USP7-mediated deubiquitinylation.