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验证数据展示

  • IF Staining of HeLa using 85048-5-RR

    IF Staining of HeLa using 85048-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PML antibody (85048-5-RR, Clone: 242564C10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of A431 using 85048-5-RR

    FC experiment of A431 using 85048-5-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug PML Recombinant antibody (85048-5-RR, Clone:242564C10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 85048-5-RR

    Affinity and Kinetic Characterization of 85048-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85048-5-RR against Human PML were performed. The affinity constant is below 1 pM.

PML Recombinant antibody

PML Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 85048-5-RR

产品说明书

CloneNo. 242564C10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

242564C10, E3 SUMO-protein ligase PML, EC:2.3.2.-, Protein PML, RING-type E3 SUMO transferase PML

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy PML Ready-To-Use IHC Kit. PML primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85048-5-RR targets PML in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 PML fusion protein Ag15377 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 promyelocytic leukemia
别名 242564C10, E3 SUMO-protein ligase PML, EC:2.3.2.-, Protein PML, RING-type E3 SUMO transferase PML
计算分子量 882 aa, 98 kDa
GenBank蛋白编号BC020994
基因名称 PML
Gene ID (NCBI) 5371
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP29590
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PML, also named as MYL, RNF71 and TRIM19, is a member of RBCC/TRIM family of proteins that possess a RING finger domain, B-box, and coiled-coil domain. It functions as tumor suppressor. PML is required for normal, caspase-dependent apoptosis in response to DNA damage, FAS, TNF, or interferons. It plays a role in transcription regulation, DNA damage response, DNA repair and chromatin organization. PML plays a role in processes regulated by retinoic acid, regulation of cell division, terminal differentiation of myeloid precursor cells and differentiation of neural progenitor cells. It regulates PTEN compartmentalization through the inhibition of USP7-mediated deubiquitinylation.

实验方案

Product Specific Protocols
IF protocol for PML antibody 85048-5-RRDownload protocol
FC protocol for PML antibody 85048-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols