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PE Anti-Mouse PLVAP (MECA-32)

PLVAP Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

MECA-32

Cat No : PE-65214

Print datasheet

Synonyms

MECA32, Plvap, PV 1

验证数据展示

  • FC experiment of bEnd.3 using PE-65214

    FC experiment of bEnd.3 using PE-65214

    1X10^6 bEnd.3 cells were surface stained with 0.1 ug PE Anti-Mouse PLVAP (PE-65214, Clone:MECA-32) (red), or 0.1 ug PE Rat IgG2a Isotype Control (2A3) (PE-65209, Clone: 2A3) (blue). Cells were not fixed.

  • FC experiment of bEnd.3 using PE-65214

    FC experiment of bEnd.3 using PE-65214

    1X10^6 bEnd.3 cells were surface stained with 0.1 ug PE Anti-Mouse PLVAP (PE-65214, Clone:MECA-32), or 0.1 ug PE Rat IgG2a Isotype Control (2A3) (PE-65209, Clone: 2A3). Cells were not fixed.

经过测试的应用

Positive FC detected inbEnd.3 cells

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-65214 targets PLVAP in FC applications and shows reactivity with mouse samples.

Tested Applications FC
Tested Reactivity mouse
Immunogen Mouse lymph node stromal cells 种属同源性预测
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Full Name plasmalemma vesicle associated protein
Synonyms MECA32, Plvap, PV 1
GenBank Accession NumberBC013517
Gene Symbol Plvap
Gene ID (NCBI) 84094
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ91VC4
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Plasmalemma vesicle-associated protein (PLVAP), also known as PV-1, PAL-E and MECA-32 antigen, is an endothelial cell-specific type II membrane N-glycosylated glycoprotein with a molecular weight of approximately 50-65 kDa, that forms homodimers in situ (PMID: 7626790; 30231925). The MECA-32 antibody, derived from rats immunized with murine lymph node stroma, shows high specificity for mouse endothelium in both embryonic and mature tissues (PMID: 2683264; 7626790). It recognizes most endothelial cells in the embryonic and in the adult mouse, with the exception of the brain, skeletal, and cardiac muscle, where the MECA-32 antigen has a more restricted distribution. In skeletal and cardiac muscle only small arterioles and venules express the MECA-32 antigen, while in the brain its expression is negatively correlated with the differentiation of the vasculature to form the blood brain barrier (PMID: 7626790).

实验方案

Product Specific Protocols
FC protocol for PE PLVAP antibody PE-65214Download protocol
Standard Protocols
Click here to view our Standard Protocols