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验证数据展示

  • IF Staining of hTERT-RPE1 using 83431-1-RR (same clone as 83431-1-PBS)

    IF Staining of hTERT-RPE1 using 83431-1-RR (same clone as 83431-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using PLK2 antibody (83431-1-RR, Clone: 230520B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red). This data was developed using the same antibody clone with 83431-1-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 83431-1-RR (same clone as 83431-1-PBS)

    FC experiment of A549 using 83431-1-RR (same clone as 83431-1-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Plk2 Recombinant Antibody (83431-1-RR, Clone:230520B2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83431-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83431-1-RR

    Affinity and Kinetic Characterization of 83431-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83431-1-RR against Human PLK2 were performed. The affinity constant is 1.53 nM.

PLK2 Recombinant monoclonal antibody, PBS Only

PLK2 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83431-1-PBS

产品说明书

CloneNo. 230520B2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

230520B2, EC:2.7.11.21, hPlk2, hSNK, PLK 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83431-1-PBS targets PLK2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag29837

Product name: Recombinant human PLK2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 357-497 aa of BC013879

Sequence: SSPAKNFFKKAAAALFGGKKDKARYIDTHNRVSKEDEDIYKLRHDLKKTSITQQPSKHRTDEELQPPTTTVARSGTPAVENKQQIGDAIRMIVRGTLGSCSSSSECLEDSTMGSVADTVARVLRGCLENMPEADCIPKEQL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 polo-like kinase 2 (Drosophila)
别名 230520B2, EC:2.7.11.21, hPlk2, hSNK, PLK 2
计算分子量 685 aa, 78 kDa
GenBank蛋白编号BC013879
基因名称 PLK2
Gene ID (NCBI) 10769
RRIDAB_3671076
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9NYY3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PLK2(Polo-like kinase 2) is also named as SNK and belongs to the Ser/Thr protein kinase family. It plays a role in normal cell division. Plk2 expression is detected only in G1, and endogenous Plk2 is rapidly turned over. Therefore, the expression of the three mammalian Plks during the cell cycle resembles the successive transition of the cyclins, prompting speculation that Plks may reg-ulate the cell cycle in a manner analogous to that of Cdks(PMID:12972611).

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