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验证数据展示

  • WB analysis of ROS1728 using 16202-1-AP

    WB analysis of ROS1728 using 16202-1-AP

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 16202-1-AP (PLEKHM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 16202-1-AP

    IHC staining of human kidney using 16202-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 16202-1-AP

    IHC staining of human kidney using 16202-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PLEKHM1 Polyclonal antibody

PLEKHM1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16202-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

162 kDa adapter protein, AP162, B2, KIAA0356, OPTB6, PLEKHM1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inROS1728 cells, mouse liver tissue
Positive IHC detected inhuman kidney tissue, human liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

16202-1-AP targets PLEKHM1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 PLEKHM1 fusion protein Ag9145 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pleckstrin homology domain containing, family M (with RUN domain) member 1
别名 162 kDa adapter protein, AP162, B2, KIAA0356, OPTB6, PLEKHM1
计算分子量 117 kDa
观测分子量 117 kDa
GenBank蛋白编号BC064361
基因名称 PLEKHM1
Gene ID (NCBI) 9842
RRIDAB_2878228
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y4G2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PLEKHM1 antibody 16202-1-APDownload protocol
IHC protocol for PLEKHM1 antibody 16202-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Autophagy

SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes

Authors - Wang Zheng
View Article
mouseWB

Nutrients

Improving Lipophagy by Restoring Rab7 Cycle: Protective Effects of Quercetin on Ethanol-Induced Liver Steatosis.

Authors - Hongkun Lin
View Article

Cell Biol Toxicol

Downregulation of MYBL1 in endothelial cells contributes to atherosclerosis by repressing PLEKHM1-inducing autophagy

Authors - Shi-Ao Ding
View Article