验证数据展示
经过测试的应用
| Positive WB detected in | ROS1728 cells, mouse liver tissue |
| Positive IHC detected in | human kidney tissue, human liver cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
16202-1-AP targets PLEKHM1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | PLEKHM1 fusion protein Ag9145 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pleckstrin homology domain containing, family M (with RUN domain) member 1 |
| 别名 | AP162, OPTB6, PH domain-containing family M member 1, Pleckstrin homology domain-containing family M member 1 |
| 计算分子量 | 117 kDa |
| 观测分子量 | 117 kDa |
| GenBank蛋白编号 | BC064361 |
| 基因名称 | PLEKHM1 |
| Gene ID (NCBI) | 9842 |
| RRID | AB_2878228 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y4G2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLEKHM1, also known as AP162, OPTB6, belongs to the Pleckstrin homology domain protein family. PLEKHM1 functions as a multivalent adapter protein that regulates Rab7-dependent and HOPS complex-dependent fusion events within the endolysosomal system, thereby coupling autophagic and endocytic trafficking pathways. In osteoclasts, PLEKHM1 is crucial for bone resorption by regulating the vesicle transport process and ensuring the formation of the ruffled border.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PLEKHM1 antibody 16202-1-AP | Download protocol |
| IHC protocol for PLEKHM1 antibody 16202-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Autophagy SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes | ||
Nutrients Improving Lipophagy by Restoring Rab7 Cycle: Protective Effects of Quercetin on Ethanol-Induced Liver Steatosis. | ||
Cell Biol Toxicol Downregulation of MYBL1 in endothelial cells contributes to atherosclerosis by repressing PLEKHM1-inducing autophagy |
