• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 85619-4-RR

    WB analysis using 85619-4-RR

    HT-29, U2OS, PC-3, Caco-2 (positive) and Ramos (negative) cells were subjected to SDS PAGE followed by western blot with 85619-4-RR (PLEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85619-4-RR

    Affinity and Kinetic Characterization of 85619-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85619-4-RR against Human PLEK2 were performed. The affinity constant is below 1 pM.

PLEK2 Recombinant antibody

PLEK2 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85619-4-RR

产品说明书

CloneNo. 242987B7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Pleckstrin 2, Pleckstrin-2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHT-29 cells, U2OS cells, PC-3 cells, Caco-2 cells
Planning an IHC experiment? We recommend our IHCeasy PLEK2 Ready-To-Use IHC Kit. PLEK2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85619-4-RR targets PLEK2 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 PLEK2 fusion protein Ag2297 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 pleckstrin 2
别名 Pleckstrin 2, Pleckstrin-2
计算分子量 353 aa, 40 kDa
观测分子量 38-40 kDa
GenBank蛋白编号BC001226
基因名称 PLEK2
Gene ID (NCBI) 26499
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NYT0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLEK2 belongs to the Pleckstrin homology domain family and is a 40-kDa protein containing the prototypic PH domains at its amino and carboxyl termini(PMID: 10419454). PLEK2 associates with membrane-bound phosphatidylinositols generated by phosphatidylinositol 3-kinase. PLEK2 interacts with the actin cytoskeleton to induce cell spreading.

实验方案

Product Specific Protocols
WB protocol for PLEK2 antibody 85619-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols