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验证数据展示

  • WB analysis using 85619-4-RR (same clone as 85619-4-PBS)

    WB analysis using 85619-4-RR (same clone as 85619-4-PBS)

    HT-29, U2OS, PC-3, Caco-2 (positive) and Ramos (negative) cells were subjected to SDS PAGE followed by western blot with 85619-4-RR (PLEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85619-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85619-4-RR

    Affinity and Kinetic Characterization of 85619-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85619-4-RR against Human PLEK2 were performed. The affinity constant is below 1 pM.

PLEK2 Recombinant monoclonal antibody, PBS Only

PLEK2 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 85619-4-PBS

产品说明书

CloneNo. 242987B7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

Pleckstrin 2, Pleckstrin-2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PLEK2 Ready-To-Use IHC Kit. PLEK2 primary antibody included.

产品信息

85619-4-PBS targets PLEK2 in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2297

Product name: Recombinant human PLEK2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 90-353 aa of BC001226

Sequence: EERDAWAFEITGAIHAGQPGKVQQLHSLRNSFKLPPHISLHRIVDKMHDSNTGIRSSPNMEQGSTYKKTFLGSSLVDWLISNSFTASRLEAVTLASMLMEENFLRPVGVRSMGAIRSGDLAEQFLDDSTALYTFAESYKKKISPKEEISLSTVELSGTVVKQGYLAKQGHKRKNWKVRRFVLRKDPAFLHYYDPSKEENRPVGGFSLRGSLVSALEDNGVPTGVKGNVQGNLFKVITKDDTHYYIQASSKAERAEWIEAIKKLT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 pleckstrin 2
别名 Pleckstrin 2, Pleckstrin-2
计算分子量 353 aa, 40 kDa
观测分子量 38-40 kDa
GenBank蛋白编号BC001226
基因名称 PLEK2
Gene ID (NCBI) 26499
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NYT0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PLEK2 belongs to the Pleckstrin homology domain family and is a 40-kDa protein containing the prototypic PH domains at its amino and carboxyl termini(PMID: 10419454). PLEK2 associates with membrane-bound phosphatidylinositols generated by phosphatidylinositol 3-kinase. PLEK2 interacts with the actin cytoskeleton to induce cell spreading.

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