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PLA2G4A Monoclonal antibody

PLA2G4A Monoclonal Antibody for WB, IF/ICC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IF/ICC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D10H8

Cat No : 68133-1-Ig

Print datasheet

Synonyms

cPLA2, cPLA2 alpha, Cytosolic phospholipase A2, PLA2G4, PLA2G4A

验证数据展示

  • WB analysis using 68133-1-Ig

    WB analysis using 68133-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 68133-1-Ig

    IHC staining of human lung cancer using 68133-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68133-1-Ig (PLA2G4A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HeLa using 68133-1-Ig

    WB analysis of HeLa using 68133-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 68133-1-Ig

    WB analysis of NIH/3T3 using 68133-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 68133-1-Ig

    WB analysis of A549 using 68133-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 68133-1-Ig

    IHC staining of human lung cancer using 68133-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68133-1-Ig (PLA2G4A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 68133-1-Ig

    IF Staining of HeLa using 68133-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PLA2G4A antibody (68133-1-Ig, Clone: 2D10H8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, NIH/3T3 cells, A549 cells, K-562 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68133-1-Ig targets PLA2G4A in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IF/ICC, IHC, ELISA
Tested Reactivity Human, mouse
Immunogen PLA2G4A fusion protein Ag32674 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phospholipase A2, group IVA (cytosolic, calcium-dependent)
Synonyms cPLA2, cPLA2 alpha, Cytosolic phospholipase A2, PLA2G4, PLA2G4A
Calculated Molecular Weight 749 aa, 85 kDa
Observed Molecular Weight85-100 kDa
GenBank Accession NumberBC114340
Gene Symbol PLA2G4A
Gene ID (NCBI) 5321
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP47712
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PLA2G4A antibody 68133-1-IgDownload protocol
IHC protocol for PLA2G4A antibody 68133-1-IgDownload protocol
IF protocol for PLA2G4A antibody 68133-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols