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Host
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Species Reactivity
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验证数据展示

  • WB analysis using 68133-1-Ig (same clone as 68133-1-PBS)

    WB analysis using 68133-1-Ig (same clone as 68133-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68133-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 68133-1-Ig (same clone as 68133-1-PBS)

    IHC staining of human lung cancer using 68133-1-Ig (same clone as 68133-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68133-1-Ig (PLA2G4A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68133-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 68133-1-Ig (same clone as 68133-1-PBS)

    WB analysis of HeLa using 68133-1-Ig (same clone as 68133-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68133-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 68133-1-Ig (same clone as 68133-1-PBS)

    WB analysis of NIH/3T3 using 68133-1-Ig (same clone as 68133-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68133-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 68133-1-Ig (same clone as 68133-1-PBS)

    WB analysis of A549 using 68133-1-Ig (same clone as 68133-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68133-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 68133-1-Ig (same clone as 68133-1-PBS)

    IHC staining of human lung cancer using 68133-1-Ig (same clone as 68133-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68133-1-Ig (PLA2G4A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68133-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68133-1-Ig (same clone as 68133-1-PBS)

    IF Staining of HeLa using 68133-1-Ig (same clone as 68133-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PLA2G4A antibody (68133-1-Ig, Clone: 2D10H8 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68133-1-PBS in a different storage buffer formulation.

PLA2G4A Monoclonal antibody, PBS Only

PLA2G4A Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68133-1-PBS

产品说明书

CloneNo. 2D10H8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

2D10H8, cPLA2, cPLA2 alpha, Lysophospholipase, Phosphatidylcholine 2-acylhydrolase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68133-1-PBS targets PLA2G4A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 PLA2G4A fusion protein Ag32674 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phospholipase A2, group IVA (cytosolic, calcium-dependent)
别名 2D10H8, cPLA2, cPLA2 alpha, Lysophospholipase, Phosphatidylcholine 2-acylhydrolase
计算分子量 749 aa, 85 kDa
观测分子量85-100 kDa
GenBank蛋白编号BC114340
基因名称 PLA2G4A
Gene ID (NCBI) 5321
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP47712
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PLA2G4A, also named cPLA2 (cytosolic phospholipase A2), is a highly conserved and widely expressed enzyme that can promote lipid mediator production. It is present in the cytosol of resting cells and upon activation by a variety of agonists, undergoes Ca++-directed translocation to perinuclear membranes.PLA2G4A plays a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response. It has been reported that the activity and expression levels of PLA2G4A are upregulated in several neurodegenerative diseases. It is a 749 amino acid, 85 kDa protein that migrates at 100-112 kDa in polyacrylamide gels(PMID: 17459165).