验证数据展示
经过测试的应用
| Positive WB detected in | pig pancreas tissue |
For other applications, we recommend the unconjugated version of this antibody, 66397-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66397 targets PLA2G1B in WB applications and shows reactivity with human, pig samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | human, pig |
| 免疫原 |
CatNo: Ag8812 Product name: Recombinant human PLA2G1B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-70 aa of BC005386 Sequence: MKLLVLAVLLTVAAADSGISPRAVWQFRKMIKCVIPGSDPFLEYNNYGCYCGLGGSGTPVDELDKQKQRV 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | phospholipase A2, group IB (pancreas) |
| 别名 | Group IB phospholipase A2, Phospholipase A2, PLA2, PLA2A, PLA2G1B, PPLA2 |
| 计算分子量 | 70aa,8 kDa; 148aa,16 kDa |
| 观测分子量 | 16 kDa |
| GenBank蛋白编号 | BC005386 |
| 基因名称 | PLA2G1B |
| Gene ID (NCBI) | 5319 |
| RRID | AB_3084243 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P04054 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 488 PLA2G1B antibody CL488-66397 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
