验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-88380 targets PIK3C2A in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag16894 Product name: Recombinant human PIK3C2A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-338 aa of BC113658 Sequence: MAQISSNSGFKECPSSHPEPTRAKDVDKEEALQMEAEALAKLQKDRQVTDNQRGFELSSSTRKKAQVYNKQDYDLMVFPESDSQKRALDIDVEKLTQAELEKLLLDDSFETKKTPVLPVTPILSPSFSAQLYFRPTIQRGQWPPGLPGPSTYALPSIYPSTYSKQAAFQNGFNPRMPTFPSTEPIYLSLPGQSPYFSYPLTPATPFHPQGSLPIYRPVVSTDMAKLFDKIASTSEFLKNGKARTDLEITDSKVSNLQVSPKSEDISKFDWLDLDPLSKPKVDNVEVLDHEEEKNVSSLLAKDPWDAVLLEERSTANCHLERKVNGKSLSVATVTRSQS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | phosphoinositide-3-kinase, class 2, alpha polypeptide |
| 别名 | 3, EC:2.7.1.137, Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha, Phosphoinositide 3-kinase-C2-alpha, PI3 K C2A |
| 计算分子量 | 1686 aa, 191 kDa |
| 观测分子量 | 191 kDa |
| GenBank蛋白编号 | BC113658 |
| 基因名称 | PIK3C2A |
| Gene ID (NCBI) | 5286 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 激发激光 | Blue laser (488 nm) |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O00443 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PIK3C2A, also known as PI3K-C2alpha, is a member of phosphatidylinositol -4- phosphate 3- kinase (PI3K) family. Genes in this family play a key role in cell signal transduction. The main function of PIK3C2A is to participate in the metabolism of phosphatidylinositol in cells, specifically, it is responsible for converting phosphatidylinositol -4- phosphate (PIP) into phosphatidylinositol -3,4- diphosphate (PIP2).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PIK3C2A antibody CL488-88380 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
