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验证数据展示

  • IF Staining of A549 using CL488-85743

    IF Staining of A549 using CL488-85743

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 PELO antibody (CL488-85743, Clone: 243137F4 ) at dilution of 1:200.

  • IF Staining of HeLa using CL488-85743

    IF Staining of HeLa using CL488-85743

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 PELO antibody (CL488-85743, Clone: 243137F4 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated PELO Recombinant monoclonal antibody

PELO Uni-rAb® Recombinant Antibody for IF/ICC
Cat No. CL488-85743

产品说明书

CloneNo. 243137F4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

CGI 17, CGI-17, hPelota, PRO1770, Protein Dom34 homolog

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells, A549 cells
Planning an IHC experiment? We recommend our IHCeasy PELO Ready-To-Use IHC Kit. PELO primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 85743-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-85743 targets PELO in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原

CatNo: Ag0873

Product name: Recombinant human PELO protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 42-384 aa of BC005889

Sequence: STIRKVQTESSTGSVGSNRVRTTLTLCVEAIDFDSQACQLRVKGTNIQENEYVKMGAYHTIELEPNRQFTLAKKQWDSVVLERIEQACDPAWSADVAAVVMQEGLAHICLVTPSMTLTRAKVEVNIPRKRKGNCSQHDRALERFYEQVVQAIQRHIHFDVVKCILVASPGFVREQFCDYMFQQAVKTDNKLLLENRSKFLQVHASSGHKYSLKEALCDPTVASRLSDTKAAGEVKALDDFYKMLQHEPDRAFYGLKQVEKANEAMAIDTLLISDELFRHQDVATRSRYVRLVDSVKENAGTVRIFSSLHVSGEQLSQLTGVAAILRFPVPELSDQEGDSSSEE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 pelota homolog (Drosophila)
别名 CGI 17, CGI-17, hPelota, PRO1770, Protein Dom34 homolog
计算分子量 43 kDa
观测分子量 43-45 kDa
GenBank蛋白编号BC005889
基因名称 PELO
Gene ID (NCBI) 53918
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9BRX2
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Pelo gene was originally identified in a mutagenesis screen for spermatogenesis-specific genes of Drosophila melanogaster. The PELO is required for the meiotic division during the G2/M transition, and functions in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs [PMID:12556505]. It may participate in the machinery of protein synthesis or in the regulation of mRNA translation [PMID:9584085].

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PELO antibody CL488-85743Download protocol
Standard Protocols
Click here to view our Standard Protocols
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