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验证数据展示

  • WB analysis using 10582-1-AP (same clone as 10582-1-PBS)

    WB analysis using 10582-1-AP (same clone as 10582-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 10582-1-AP (PELO antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse testis using 10582-1-AP (same clone as 10582-1-PBS)

    WB analysis of mouse testis using 10582-1-AP (same clone as 10582-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 10582-1-AP (PELO antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 10582-1-AP (same clone as 10582-1-PBS)

    IHC staining of human liver using 10582-1-AP (same clone as 10582-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PELO antibody included
  • IP experiment of HEK-293 using 10582-1-AP (same clone as 10582-1-PBS)

    IP experiment of HEK-293 using 10582-1-AP (same clone as 10582-1-PBS)

    IP result of anti-PELO (IP:10582-1-AP, 4ug; Detection:10582-1-AP 1:500) with HEK-293 cells lysate 1200ug. This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

  • WB analysis using 10582-1-AP (same clone as 10582-1-PBS)

    WB analysis using 10582-1-AP (same clone as 10582-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10582-1-AP (PELO antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse stomach using 10582-1-AP (same clone as 10582-1-PBS)

    IHC staining of mouse stomach using 10582-1-AP (same clone as 10582-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PELO antibody included
  • IHC staining of human ovary cancer using 10582-1-AP (same clone as 10582-1-PBS)

    IHC staining of human ovary cancer using 10582-1-AP (same clone as 10582-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PELO antibody included
  • IHC staining of mouse pancreas using 10582-1-AP (same clone as 10582-1-PBS)

    IHC staining of mouse pancreas using 10582-1-AP (same clone as 10582-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PELO antibody included
  • IHC staining of rat ovary using 10582-1-AP (same clone as 10582-1-PBS)

    IHC staining of rat ovary using 10582-1-AP (same clone as 10582-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PELO antibody included
  • IF Staining of HeLa using 10582-1-AP (same clone as 10582-1-PBS)

    IF Staining of HeLa using 10582-1-AP (same clone as 10582-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PELO antibody (10582-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PELO Polyclonal antibody, PBS Only

PELO Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 10582-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

CGI 17, CGI-17, hPelota, PRO1770, Protein Dom34 homolog

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PELO Ready-To-Use IHC Kit. PELO primary antibody included.

产品信息

10582-1-PBS targets PELO in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0873

Product name: Recombinant human PELO protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 42-384 aa of BC005889

Sequence: STIRKVQTESSTGSVGSNRVRTTLTLCVEAIDFDSQACQLRVKGTNIQENEYVKMGAYHTIELEPNRQFTLAKKQWDSVVLERIEQACDPAWSADVAAVVMQEGLAHICLVTPSMTLTRAKVEVNIPRKRKGNCSQHDRALERFYEQVVQAIQRHIHFDVVKCILVASPGFVREQFCDYMFQQAVKTDNKLLLENRSKFLQVHASSGHKYSLKEALCDPTVASRLSDTKAAGEVKALDDFYKMLQHEPDRAFYGLKQVEKANEAMAIDTLLISDELFRHQDVATRSRYVRLVDSVKENAGTVRIFSSLHVSGEQLSQLTGVAAILRFPVPELSDQEGDSSSEE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pelota homolog (Drosophila)
别名 CGI 17, CGI-17, hPelota, PRO1770, Protein Dom34 homolog
计算分子量 43 kDa
观测分子量 43-45 kDa
GenBank蛋白编号BC005889
基因名称 PELO
Gene ID (NCBI) 53918
RRIDAB_2236833
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BRX2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The Pelo gene was originally identified in a mutagenesis screen for spermatogenesis-specific genes of Drosophila melanogaster. The PELO is required for the meiotic division during the G2/M transition, and functions in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs [PMID:12556505]. It may participate in the machinery of protein synthesis or in the regulation of mRNA translation [PMID:9584085].

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