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验证数据展示

  • WB analysis using 10582-1-AP

    WB analysis using 10582-1-AP

    Various cells were subjected to SDS PAGE followed by western blot with 10582-1-AP (PELO antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse testis using 10582-1-AP

    WB analysis of mouse testis using 10582-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 10582-1-AP (PELO antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 10582-1-AP

    IHC staining of human liver using 10582-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PELO antibody included
  • IP experiment of HEK-293 using 10582-1-AP

    IP experiment of HEK-293 using 10582-1-AP

    IP result of anti-PELO (IP:10582-1-AP, 4ug; Detection:10582-1-AP 1:500) with HEK-293 cells lysate 1200ug.

  • WB analysis using 10582-1-AP

    WB analysis using 10582-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10582-1-AP (PELO antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse stomach using 10582-1-AP

    IHC staining of mouse stomach using 10582-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PELO antibody included
  • IHC staining of mouse stomach using 10582-1-AP

    IHC staining of mouse stomach using 10582-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PELO antibody included
  • IHC staining of human ovary cancer using 10582-1-AP

    IHC staining of human ovary cancer using 10582-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PELO antibody included
  • IHC staining of human ovary cancer using 10582-1-AP

    IHC staining of human ovary cancer using 10582-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PELO antibody included
  • IHC staining of human placenta using 10582-1-AP

    IHC staining of human placenta using 10582-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PELO antibody included
  • IHC staining of mouse ovary using 10582-1-AP

    IHC staining of mouse ovary using 10582-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PELO antibody included
  • IHC staining of mouse pancreas using 10582-1-AP

    IHC staining of mouse pancreas using 10582-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PELO antibody included
  • IHC staining of rat ovary using 10582-1-AP

    IHC staining of rat ovary using 10582-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PELO antibody included
  • IHC staining of rat ovary using 10582-1-AP

    IHC staining of rat ovary using 10582-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PELO antibody included
  • IHC staining of rat stomach using 10582-1-AP

    IHC staining of rat stomach using 10582-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PELO antibody included
  • IHC staining of mouse stomach using 10582-1-AP

    IHC staining of mouse stomach using 10582-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PELO antibody included
  • IF Staining of HeLa using 10582-1-AP

    IF Staining of HeLa using 10582-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PELO antibody (10582-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Featured Product
  • KD/KO Validated

PELO Polyclonal antibody

PELO Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10582-1-AP

产品说明书

引用文献(19)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

CGI 17, CGI-17, hPelota, PRO1770, Protein Dom34 homolog

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, mouse testis tissue, SKOV-3 cells, mouse kidney tissue, rat kidney tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman ovary cancer tissue, rat stomach tissue, human liver tissue, mouse pancreas tissue, rat ovary tissue, human placenta tissue, mouse ovary tissue, mouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PELO Ready-To-Use IHC Kit. PELO primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10582-1-AP targets PELO in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag0873

Product name: Recombinant human PELO protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 42-384 aa of BC005889

Sequence: STIRKVQTESSTGSVGSNRVRTTLTLCVEAIDFDSQACQLRVKGTNIQENEYVKMGAYHTIELEPNRQFTLAKKQWDSVVLERIEQACDPAWSADVAAVVMQEGLAHICLVTPSMTLTRAKVEVNIPRKRKGNCSQHDRALERFYEQVVQAIQRHIHFDVVKCILVASPGFVREQFCDYMFQQAVKTDNKLLLENRSKFLQVHASSGHKYSLKEALCDPTVASRLSDTKAAGEVKALDDFYKMLQHEPDRAFYGLKQVEKANEAMAIDTLLISDELFRHQDVATRSRYVRLVDSVKENAGTVRIFSSLHVSGEQLSQLTGVAAILRFPVPELSDQEGDSSSEE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pelota homolog (Drosophila)
别名 CGI 17, CGI-17, hPelota, PRO1770, Protein Dom34 homolog
计算分子量 43 kDa
观测分子量 43-45 kDa
GenBank蛋白编号BC005889
基因名称 PELO
Gene ID (NCBI) 53918
RRIDAB_2236833
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BRX2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Pelo gene was originally identified in a mutagenesis screen for spermatogenesis-specific genes of Drosophila melanogaster. The PELO is required for the meiotic division during the G2/M transition, and functions in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs [PMID:12556505]. It may participate in the machinery of protein synthesis or in the regulation of mRNA translation [PMID:9584085].

实验方案

Product Specific Protocols
IF protocol for PELO antibody 10582-1-APDownload protocol
IHC protocol for PELO antibody 10582-1-APDownload protocol
IP protocol for PELO antibody 10582-1-APDownload protocol
WB protocol for PELO antibody 10582-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

Synthetic lethality of mRNA quality control complexes in cancer

Authors - Vivian Prindle
  • KO Validated
View Article
humanWB

Cell

Global genetic interaction network of a human cell maps conserved principles and informs functional interpretation of gene co-essentiality profiles.

Authors - Maximilian Billmann
  • KO Validated
View Article
mouseWB

Immunity

Ribosome-rescuer PELO catalyzes the oligomeric assembly of NOD-like receptor family proteins via activating their ATPase enzymatic activity

Authors - Xiurong Wu
View Article
humanWB

Nat Commun

Single-molecule imaging reveals distinct elongation and frameshifting dynamics between frames of expanded RNA repeats in C9ORF72-ALS/FTD

Authors - Malgorzata J Latallo
View Article
WB

Nat Commun

Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis

Authors - Ji Geng
View Article
humanWB

Mol Cell

Unresolved stalled ribosome complexes restrict cell-cycle progression after genotoxic stress.

Authors - Mark Stoneley
View Article
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