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验证数据展示

  • WB analysis using 85027-5-RR (same clone as 85027-5-PBS)

    WB analysis using 85027-5-RR (same clone as 85027-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85027-5-RR (PDLIM1,CLP36 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85027-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 85027-5-RR (same clone as 85027-5-PBS)

    IF Staining of HeLa using 85027-5-RR (same clone as 85027-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PDLIM1/CLP36 antibody (85027-5-RR, Clone: 242585C7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85027-5-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 85027-5-RR (same clone as 85027-5-PBS)

    FC experiment of HepG2 using 85027-5-RR (same clone as 85027-5-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PDLIM1/CLP36 Recombinant antibody (85027-5-RR, Clone:242585C7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85027-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85027-5-RR

    Affinity and Kinetic Characterization of 85027-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85027-5-RR against Human PDLIM1/CLP36 were performed. The affinity constant is 6.23 nM.

PDLIM1/CLP36 Recombinant antibody, PBS Only

PDLIM1/CLP36 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 85027-5-PBS

产品说明书

CloneNo. 242585C7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

PDLIM1, 242585C7, CLIM1, CLP36, CLP-36

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

85027-5-PBS targets PDLIM1/CLP36 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PDLIM1/CLP36 fusion protein Ag2274 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 PDZ and LIM domain 1
别名 PDLIM1, 242585C7, CLIM1, CLP36, CLP-36
计算分子量 329 aa, 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC000915
基因名称 PDLIM1/CLP36
Gene ID (NCBI) 9124
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO00151
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PDLIM1, also named as CLIM1, CLP-36, CLP36, hCLIM1 and Elfin, is a member of the actinin-associated LIM protein (ALP)/enigma protein family, plays a role in neurite outgrowth in the peripheral nervous system. It is a cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton. PDLIM1 and PALLD formed a complex in the dorsal root ganglion (DRG) and PDLIM1 and PALLD were colocalized in the neurites and cell bodies of primary DRG neurons. PDLIM1 may interact with PALLD to influence neurite outgrowth during sciatic nerve regeneration. (PMID: 20381583)