• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 87880-1-RR

    WB analysis using 87880-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87880-1-RR (PDHX antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Affinity of 87880-1-RR

    Affinity of 87880-1-RR

    Biolayer interferometry (BLI) kinetic assays of 87880-1-RR against Human PDHX were performed. The affinity constant is below 1 pM.

PDHX Recombinant monoclonal antibody

PDHX Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 87880-1-RR

产品说明书

CloneNo. 253557E3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

PDX1, Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex, E3 binding protein, E3-binding protein, Lipoyl-containing pyruvate dehydrogenase complex component X

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHepG2 cells, BxPC-3 cells, HEK-293 cells, SKOV-3 cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy PDHX Ready-To-Use IHC Kit. PDHX primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87880-1-RR targets PDHX in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag21034

Product name: Recombinant human PDHX protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-501 aa of BC010389

Sequence: MAASWRLGCDPRLLRYLVGFPGRRSVGLVKGALGWSVSRGANWRWFHSTQWLRGDPIKILMPSLSPTMEEGNIVKWLKKEGEAVSAGDALCEIETDKAVVTLDASDDGILAKIVVEEGSKNIRLGSLIGLIVEEGEDWKHVEIPKDVGPPPPVSKPSEPRPSPEPQISIPVKKEHIPGTLRFRLSPAARNILEKHSLDASQGTATGPRGIFTKEDALKLVQLKQTGKITESRPTPAPTATPTAPSPLQATAGPSYPRPVIPPVSTPGQPNAVGTFTEIPASNIRRVIAKRLTESKSTVPHAYATADCDLGAVLKVRQDLVKDDIKVSVNDFIIKAAAVTLKQMPDVNVSWDGEGPKQLPFIDISVAVATVKGLLTPIIKDAAAKGIQEIADSVKALSKKARDGKLLPEEYQGGSFSISNLGMFGIDEFTAVINPPQACILAVGRFRPVLKLTEDEEGNAKLQQRQLITVTMSSDSRVVDDELATRFLKSFKANLENPIRLA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 pyruvate dehydrogenase complex, component X
别名 PDX1, Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex, E3 binding protein, E3-binding protein, Lipoyl-containing pyruvate dehydrogenase complex component X
计算分子量 54 kDa
观测分子量54 kDa
GenBank蛋白编号BC010389
基因名称 PDHX
Gene ID (NCBI) 8050
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO00330
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDHX (Pyruvate dehydrogenase protein X component), an E3-binding protein in the PDC, is acetylated by the p300 at Lys 488, impeding the interaction between PDHX and dihydrolipoyl transacetylase (E2), thereby disrupting PDC assembly to inhibit its activation (PMID: 30012170). PDHX was characterized as a metabolically essential gene for esophageal squamous cell carcinoma (ESCC), and knockdown of PDHX inhibited the proliferation of cancer stem cells (CSCs) and in vivo tumor growth (PMID: 33964039). Western blot analysis detected PDHX at an apparent molecular mass of 54 kDa.

实验方案

Product Specific Protocols
WB protocol for PDHX antibody 87880-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...