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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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验证数据展示

  • WB analysis using 87880-1-RR (same clone as 87880-1-PBS)

    WB analysis using 87880-1-RR (same clone as 87880-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87880-1-RR (PDHX antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87880-1-PBS in a different storage buffer formulation.

  • Affinity of 87880-1-RR

    Affinity of 87880-1-RR

    Biolayer interferometry (BLI) kinetic assays of 87880-1-RR against Human PDHX were performed. The affinity constant is below 1 pM.

PDHX Recombinant monoclonal antibody, PBS Only

PDHX Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 87880-1-PBS

产品说明书

CloneNo. 253557E3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Indirect ELISA

别名

PDX1, Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex, E3 binding protein, E3-binding protein, Lipoyl-containing pyruvate dehydrogenase complex component X

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PDHX Ready-To-Use IHC Kit. PDHX primary antibody included.

产品信息

87880-1-PBS targets PDHX in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag21034

Product name: Recombinant human PDHX protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-501 aa of BC010389

Sequence: MAASWRLGCDPRLLRYLVGFPGRRSVGLVKGALGWSVSRGANWRWFHSTQWLRGDPIKILMPSLSPTMEEGNIVKWLKKEGEAVSAGDALCEIETDKAVVTLDASDDGILAKIVVEEGSKNIRLGSLIGLIVEEGEDWKHVEIPKDVGPPPPVSKPSEPRPSPEPQISIPVKKEHIPGTLRFRLSPAARNILEKHSLDASQGTATGPRGIFTKEDALKLVQLKQTGKITESRPTPAPTATPTAPSPLQATAGPSYPRPVIPPVSTPGQPNAVGTFTEIPASNIRRVIAKRLTESKSTVPHAYATADCDLGAVLKVRQDLVKDDIKVSVNDFIIKAAAVTLKQMPDVNVSWDGEGPKQLPFIDISVAVATVKGLLTPIIKDAAAKGIQEIADSVKALSKKARDGKLLPEEYQGGSFSISNLGMFGIDEFTAVINPPQACILAVGRFRPVLKLTEDEEGNAKLQQRQLITVTMSSDSRVVDDELATRFLKSFKANLENPIRLA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 pyruvate dehydrogenase complex, component X
别名 PDX1, Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex, E3 binding protein, E3-binding protein, Lipoyl-containing pyruvate dehydrogenase complex component X
计算分子量 54 kDa
观测分子量54 kDa
GenBank蛋白编号BC010389
基因名称 PDHX
Gene ID (NCBI) 8050
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO00330
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PDHX (Pyruvate dehydrogenase protein X component), an E3-binding protein in the PDC, is acetylated by the p300 at Lys 488, impeding the interaction between PDHX and dihydrolipoyl transacetylase (E2), thereby disrupting PDC assembly to inhibit its activation (PMID: 30012170). PDHX was characterized as a metabolically essential gene for esophageal squamous cell carcinoma (ESCC), and knockdown of PDHX inhibited the proliferation of cancer stem cells (CSCs) and in vivo tumor growth (PMID: 33964039). Western blot analysis detected PDHX at an apparent molecular mass of 54 kDa.

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