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验证数据展示

  • WB analysis using 17134-1-AP (same clone as 17134-1-PBS)

    WB analysis using 17134-1-AP (same clone as 17134-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17134-1-AP (PDHA2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17134-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 17134-1-AP (same clone as 17134-1-PBS)

    IHC staining of mouse testis using 17134-1-AP (same clone as 17134-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17134-1-AP (PDHA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17134-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PDHA2 antibody included

PDHA2/PDHA1 Polyclonal antibody, PBS Only

PDHA2/PDHA1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 17134-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

PDHA2, EC:1.2.4.1, PDHA 2, PDHAL, PDHE1 A type II

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PDHA2 Ready-To-Use IHC Kit. PDHA2 primary antibody included.

产品信息

17134-1-PBS targets PDHA2/PDHA1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10882

Product name: Recombinant human PDHA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-388 aa of BC030697

Sequence: MLAAFISRVLRRVAQKSARRVLVASRNSSNDATFEIKKCDLYLLEEGPPVTTVLTRAEGLKYYRMMLTVRRMELKADQLYKQKFIRGFCHLCDGQEACCVGLEAGINPSDHVITSYRAHGVCYTRGLSVRSILAELTGRRGGCAKGKGGSMHMYTKNFYGGNGIVGAQGPLGAGIALACKYKGNDEICLTLYGDGAANQGQIAEAFNMAALWKLPCVFICENNLYGMGTSTERAAASPDYYKRGNFIPGLKVDGMDVLCVREATKFAANYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKRDPIIILQDRMVNSKLATVEELKEIGAEVRKEIDDAAQFATTDPEPHLEELGHHIYSSDSSFEVRGANPWIKFKSVS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pyruvate dehydrogenase (lipoamide) alpha 2
别名 PDHA2, EC:1.2.4.1, PDHA 2, PDHAL, PDHE1 A type II
计算分子量 388 aa, 43 kDa
观测分子量 40-43 kDa
GenBank蛋白编号BC030697
基因名称 PDHA2
Gene ID (NCBI) 5161
RRIDAB_2878352
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP29803
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PDHA2 (Pyruvate Dehydrogenase E1 Subunit Alpha 2) is a testis-specific mitochondrial enzyme encoded by the PDHA2 gene on human chromosome 4q22.3. The gene exhibits 84% nucleotide sequence similarity with the X-linked PDHA1, supporting the hypothesis that PDHA2 originated through retrotransposition of PDHA1 to an autosomal location. This antibody can recognize both PDHA2 and PDHA1.

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